Fig. 1
From: Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells
Expression of free fatty acid receptor 4 (FFAR4) in bovine ovarian follicles by immunohistochemistry. Immunohistochemistry was performed on sections of ovarian follicles. FFAR4 (brown labeling) was immunodetected in ovarian follicles with large (FLA) or small (FSA) antrum (customized FFAR4 rabbit antibody, Agro-Bio). Pre-immunized rabbit serum was used as the control with the same secondary antibody as for FFAR4 detection. Bars = 200 μm and 50 μm for 10× and 40× microscope objectives, respectively. FF - follicular fluid, GC – granulosa cells, TC – theca cells