Table 1 Primer sequences of rat targeted genes
Targeted Genes | Primer sequence | Accession number |
|---|---|---|
GnRH1 | F: 5’ CCGCTGTTGTTCTGTTGACTG 3′ R: 5’ TCACACTCGGATGTTGTGGA 3′ | NM_012767 |
GnRHR | F: 5’ TCTGCAATGCCAAAATCATC 3′ R: 5’ GTAGGGAGTCCAGCAGATGAC 3′ | NM_031038.3 |
FSHβ | F: 5’ AGGAAGAGTGCCGTTTCTGC 3′ R: 5’ GCTGTCACTATCACACTTGC 3′ | NM_001007597.2 |
LHβ | F: 5’ CTGTCCTAGCATGGTTCGAGT 3′ R: 5’ AGTTAGTGGGTGGGCATCAG 3′ | NM_012858.2 |
TH | F: 5’ CATTGGACTTGCATCTCTGG 3′ R: 5’ GTTCCTGAGCTTGTCCTTGG 3′ | NM_012740.3 |
COMT | F: 5’ GACGCGAAAGGCCAAATCAT 3′ R: 5’ ACGTTGTCAGCTAGGAGCA 3′ | NM_012531.2 |
5HT1A | F:5’CCCCCCAAGAAGAGCCTGAA3′ R:5’GGCAGCCAGCAGAGGATGAA3′ | NM_012585.1 |
α1AR | F: 5’ ACCAGCTCCGGTGAACATTT 3′ R: 5’ GCCGCCCAGATATTGCAGAA 3′ | NM_017191.2 |
D2R | F:5’ TGAACCTGTGTGCCATCAGCA 3′ R:5’ TTGGCTCTGAAAGCTCGACTG 3′ | NM_012547.1 |
GABAB1 | F:5’CGCTACCATCCAACAGACCA3′ R:5’TGTCAGCATACCACCCGATG3′ | NM_031028.3 |
M2-AchR | F:5’CACAGTTTCCACTTCGCTGG 3′ R:5’ CACCTTTTTGGGCCTTGGTG 3′ | NM_031016.1 |
NMDAR | F:5’ ACACCGACCAAGAAGCCATC 3′ R:5’ GGACTCATCCTTATCCGCCA 3 | NM_012574.1 |
β-Actin | F: 5’ AGGCCCCTCTGAACCCTAAG 3′ R: 5’ GGAGCGCGTAACCCTCATAG 3′ | NM_031144.3 |