Fig. 1

Validation of PCOS rat model after 21 days of letrozole treatment. Hematoxylin-Eosin stained ovarian sections of Control group (a) and letrozole treatment group (b) under 4X magnification (Scale bar = 500μm). PF: Primary follicle; SF: secondary follicle; CL: Corpus luteum; C: Cyst. c Number of cystic follicles, d number of CL, and e percent of females that were acyclic after letrozole treatment. Change in body weight (f); Oral glucose tolerance test profile (g) and area under the curve (AUC) for glucose tolerance (h) in control and treated rats. Error bars represent SEM; n = 6–10 per group; **P < 0.01; ***P < 0.001 as compared to control group