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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Allopregnanolone alters follicular and luteal dynamics during the estrous cycle

Fig. 4

Effect of ALLO on ovarian relative vascular area. Representative photomicrographs of ovaries immunostained for α-actin (a) and von Willebrand factor (b) at each stage of the estrous cycle. α-actin control groups (a upper panel), α-actin ALLO treated groups (a lower panel); von Willebrand factor control groups (b upper panel), von Willebrand factor ALLO treated groups (b lower panel). Values are expressed as mean ± S.E.M of the relative vascular area. Two-way ANOVA followed by Bonferroni’s post hoc (n=6), *p<0.05, ***p<0.001. Bar = 50 μm. (c) Representative photomicrographs of von Willebrand factor immunostaining in corpora lutea of control (c, upper panel) and ALLO treated animals (c, lower panel). Inset shows higher magnification images (40x). Arrows indicate von Willebrand factor staining of endothelial cells. Bar = 50 μm

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