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Table 1 Primers for real-time qPCR

From: Excessive nerve growth factor impairs bidirectional communication between the oocyte and cumulus cells resulting in reduced oocyte competence

Gene name GenBank accession number Sequences of forward (F) and reverse (R) primers Amplification size (bp)
m-Pfkp NM_019703 F: 5′-GCCGTGAAACTCCGAGGAA-3′
R: 5′-GTTGCTCTTGACAATCTTCTCATCAG-3′
96
m-Ldha NM_001136069.2 F: 5′-TGTGGCAGACTTGGCTGAGA-3′
R: 5′-CTGAGGAAGACATCCTCATTGATTC-3′
105
m-Gdf9 NM_008110.2 F: 5′-TCACCTCTACAATACCGTCCGG-3′
R: 5′-GAGCAAGTGTTCCATGGCAGTC-3′
139
m-Bmp15 NM_009757.4 F: 5′-GCACGATTGGAGCGAAAATG-3′
R: 5′-CGTACGCTACCTGGTTTGATGC-3′
123
m-Fgf8 NM_010205.2 F: 5′-CAGGTCTCTACATCTGCATGAACAA-3′
R: 5′-TCTCCAGCACGATCTCTGTGAATA-3′
96
m-Actin NM_007393.5 F:5′-TGGCTCCTAGCACCATGAA-3′
R: 5′-CTCAGTAACAGTCCGCCTAGAAGCA-3′
186
h-PFKP NM_002627.4 F: 5′-AGGCGATGGACGAGAGGAGAT-3′
R: 5′-TGATGGCAAGTCGCTTGTAG-3′
93
h-LDHA NM_001165414.1 F: 5′-TGCACCCAGATTTAGGGACTGAT-3′
R: 5′-CCCAGGATGTGTAGCCTTTGAG-3′
111
h-ACTIN NM_001101.3 F: 5′-TGGCACCCAGCACAATGAA-3′
R: 5′-CTAAGTCATAGTCCGCCTAGAAGCA-3′
186
  1. m- mouse, h- human, bp base pair