Table 1 Primers for real-time qPCR
Gene name | GenBank accession number | Sequences of forward (F) and reverse (R) primers | Amplification size (bp) |
|---|---|---|---|
m-Pfkp | NM_019703 | F: 5′-GCCGTGAAACTCCGAGGAA-3′ R: 5′-GTTGCTCTTGACAATCTTCTCATCAG-3′ | 96 |
m-Ldha | NM_001136069.2 | F: 5′-TGTGGCAGACTTGGCTGAGA-3′ R: 5′-CTGAGGAAGACATCCTCATTGATTC-3′ | 105 |
m-Gdf9 | NM_008110.2 | F: 5′-TCACCTCTACAATACCGTCCGG-3′ R: 5′-GAGCAAGTGTTCCATGGCAGTC-3′ | 139 |
m-Bmp15 | NM_009757.4 | F: 5′-GCACGATTGGAGCGAAAATG-3′ R: 5′-CGTACGCTACCTGGTTTGATGC-3′ | 123 |
m-Fgf8 | NM_010205.2 | F: 5′-CAGGTCTCTACATCTGCATGAACAA-3′ R: 5′-TCTCCAGCACGATCTCTGTGAATA-3′ | 96 |
m-Actin | NM_007393.5 | F:5′-TGGCTCCTAGCACCATGAA-3′ R: 5′-CTCAGTAACAGTCCGCCTAGAAGCA-3′ | 186 |
h-PFKP | NM_002627.4 | F: 5′-AGGCGATGGACGAGAGGAGAT-3′ R: 5′-TGATGGCAAGTCGCTTGTAG-3′ | 93 |
h-LDHA | NM_001165414.1 | F: 5′-TGCACCCAGATTTAGGGACTGAT-3′ R: 5′-CCCAGGATGTGTAGCCTTTGAG-3′ | 111 |
h-ACTIN | NM_001101.3 | F: 5′-TGGCACCCAGCACAATGAA-3′ R: 5′-CTAAGTCATAGTCCGCCTAGAAGCA-3′ | 186 |