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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Excessive nerve growth factor impairs bidirectional communication between the oocyte and cumulus cells resulting in reduced oocyte competence

Fig. 4

Down-regulation of Pfkp and Ldha were dependent on oocytes. a Relative mRNA levels of Pfkp and Ldha in CCs from OOX-cumulus cells or CCs/COCs were determined by real-time qPCR in the presence or absence of NGF. CC/OOX, OOX-cumulus cells group; CCs/COC, cumulus cells from the COC group. b Relative mRNA levels of Gdf9, Bmp15 and Fgf8 in oocytes/COCs cultured with or without 100 ng/ml NGF for 18 h were determined by real-time qPCR. ≥35 samples were used in each group. Data of three independent experiments are presented as mean ± SEM. The student’s t-test was used for statistical analysis *, p < 0.05; **, p < 0.01

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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