Fig. 3

Down-regulation of Pfkp and Ldha in CCs/COCs occurred in both mice and humans. a Relative mRNA levels of Pfkp and Ldha in mouse CCs/COCs were determined by real-time qPCR, after culturing with 100 ng/ml NGF for 18 h. ≥35 COCs in each group were used and experiments were repeated three times. b Relative mRNA levels of PFKP and LDHA in CCs from non-PCOS (n = 9) patients and PCOS patients (n = 5) were determined by real-time qPCR. Data are presented as mean ± SEM. The student’s t-test was used for statistical analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001