Fig. 3

Hematoxylin and eosin staining of bovine ovarian tissues from chorioallantoic membrane (CAM) and in vitro cultures on day (d) 0, 1, 3 and 5. a Histological section of CAM cultured bovine ovarian tissue on day 0 showing several follicles. Upper right insert represents an enlarged image of two healthy growing [HG] and one atretic primordial [AP] follicle. Upper left insert represents an enlarged image of an atretic primordial [AP] follicle. Scale bar = 50 μm. Insert scale bar = 10 μm. b Histological section of CAM cultured bovine ovarian tissue on day 1 showing several follicles. Upper right insert represents an enlarged image of an atretic primordial [AP] follicle. Scale bar = 50 μm. Insert scale bar = 10 μm. c Histological section of CAM cultured bovine ovarian tissue on day 3 showing several follicles. Upper right insert represents an enlarged image of healthy growing [HG] and atretic growing [AG] follicles. Scale bar = 50 μm. Insert scale bar = 10 μm. d Histological section of CAM cultured bovine ovarian tissue on day 5 showing two follicles. Upper right insert represents an enlarged image of an atretic growing [AG] follicle. Scale bar = 50 μm. Insert scale bar = 10 μm. e Histological section of in vitro cultured bovine ovarian tissue on day 0 showing few follicles. Upper right insert represents an enlarged image of an atretic growing AG] follicle. Upper left insert represents an enlarged image of a healthy primordial [HP] follicle. Scale bar = 50 μm. Insert scale bar = 10 μm. f Histological section of in vitro cultured bovine ovarian tissue on day 1 showing few follicles. Upper right insert represents an enlarged image of an atretic growing [AG] follicle. Scale bar = 50 μm. Insert scale bar = 10 μm. g Histological section of in vitro cultured bovine ovarian tissue on day 3 showing few follicles. Upper right insert represents an enlarged image of an atretic primordial [AP] follicle. Scale bar = 50 μm. Insert scale bar = 10 μm. h Histological section of in vitro cultured bovine ovarian tissue on day 5 showing few follicles. Upper right insert represents an enlarged image of an atretic growing [AG] follicle. Scale bar = 50 μm. Insert scale bar = 10 μm