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Table 3 Effects of short-term lactate treatment on gene expression of selected marker genes and steroid hormone production

From: Lactate promotes specific differentiation in bovine granulosa cells depending on lactate uptake thus mimicking an early post-LH stage

Gene / hormone CYP19A1 FSHR LHCGR RGS2 VNN2 E2 P4
Control 3.12 (± 0.31) 34.70 (± 4.41) 3.29 (± 0.46) 5.56 (± 0.29) 6.26 (± 0.39) 23.72 (± 1.57) 824.61 (± 84.99)
L-lactate 3.78 (± 0.85) 49.10 (± 8.33) 3.18 (± 0.94) 5.78 (± 0.32) 7.39 (± 1.11) 32.31 (± 5.26) 905.01 (± 47.03)
P 0.50 0.20 0.92 0.63 0.39 0.19 0.45
  1. GC were stimulated with 30 mM L-lactate or the vehicle control NaCl 24 h prior to the end of the culture period of 8 days. Transcript abundance (×10−3, normalized to the reference gene RPLP0) and hormone concentrations (in ng/ml) are shown as means (± SEM) of n = 3 replicates, P-values were calculated using the student’s t-test or Mann-Whitney Test