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Table 3 Effects of short-term lactate treatment on gene expression of selected marker genes and steroid hormone production

From: Lactate promotes specific differentiation in bovine granulosa cells depending on lactate uptake thus mimicking an early post-LH stage

Gene / hormone

CYP19A1

FSHR

LHCGR

RGS2

VNN2

E2

P4

Control

3.12 (± 0.31)

34.70 (± 4.41)

3.29 (± 0.46)

5.56 (± 0.29)

6.26 (± 0.39)

23.72 (± 1.57)

824.61 (± 84.99)

L-lactate

3.78 (± 0.85)

49.10 (± 8.33)

3.18 (± 0.94)

5.78 (± 0.32)

7.39 (± 1.11)

32.31 (± 5.26)

905.01 (± 47.03)

P

0.50

0.20

0.92

0.63

0.39

0.19

0.45

  1. GC were stimulated with 30 mM L-lactate or the vehicle control NaCl 24 h prior to the end of the culture period of 8 days. Transcript abundance (×10−3, normalized to the reference gene RPLP0) and hormone concentrations (in ng/ml) are shown as means (± SEM) of n = 3 replicates, P-values were calculated using the student’s t-test or Mann-Whitney Test