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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Lactate promotes specific differentiation in bovine granulosa cells depending on lactate uptake thus mimicking an early post-LH stage

Fig. 5

Effect of L-lactate and specific inhibitor of L-lactate transport on genes involved in L-lactate metabolism. The abundance of LDHA and SLC16A1 transcripts was up-regulated in L-lactate treated GC (grey bars) compared to the control (dashed line; *). When the cells were pre-incubated with the inhibitors UK5099 the L-lactate induced changes of expression levels were largely abolished. No effect was observed with the inhibitor alone (light grey bars). Transcript abundance was normalized to RPLP0 as a reference gene. Means and SEM are shown of n = 4 replicates, *P < 0.05, student’s t-test or Mann-Whitney test was uses to test treatment effects compared to the vehicle control (*) and between different treatments the ANOVA was used (indicated by letters).

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