Fig. 3
From: Expression of cystatin C in the female reproductive tract and its effect on human sperm capacitation
CST3 binding to human sperm. Sperm isolated using the swim-up method was smeared onto slides for immunohistochemical staining (a and b). Sperm isolated using PureSperm density gradient were incubated without (c) or with (d) CST3. After washing off unbound CST3, sperm was transferred onto slides and fixed with 4% (w/v) paraformaldehyde. Slides were incubated with control antiserum (a) or anti-CST3 antibody (b–d) diluted 1:250, treated with FITC-conjugated goat anti-rabbit IgG, and then counterstained with Hoechst dye to localize the nuclei for contrast. Symbols: mp, midpiece; pa, post-acrosome; pp., principal piece. Bar: 10 μm