Study aim | Carnitine dose | Study design/Subjects | Outcomes | Reference |
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LC on antagonizing the harmful effect of TNF-α, apoptosis, and oxidative stress on mouse embryo development. | LC was dissolved in HTF culture medium in concentrations of 0.3 and 0.6 mg/mL | • 500 mouse embryos were divided into three groups and incubated with either AD 0.005 mg/mL, H2O2 500 mmol/L, or TNF-α 500 ng with and without LC 0.3 or 0.6 mg/mL • For anti-apoptotic effect: All groups were incubated at 37C in 5%CO2 for 4 h and transferred to HTF medium and incubated until 48 h for formation of the blastocyst stage. • For anti-oxidant and anti-proliferative effect on TNF-α: All groups were incubated at 37C in 5% CO2 for 72 h until the formation of blastocyst stage. • Embryo staining by TUNEL was done to detect blastomere DNA damage | • Significant improvement in percentage BDR was seen at LC 0.3 mg/mL compared with the control (p < 0.006) • L-Carnitine at 0.3 and 0.6 mg/mL significantly reduced the blocking effect of AD, H2O2, and TNF-α and significantly decreased the level of DNA damage | [6] |
LC on oocyte cytoskeleton and apoptosis in peritoneal fluid from patients with endometriosis | 0.6 mg/mL of LC | • Peritoneal fluid was collected from 23 women suffering from endometriosis and 15 patients with tubal ligation who underwent laparoscopy • LC was diluted 1:1 with peritoneal fluid and cryopreserved mouse embryos were matured in that medium | Significantly improved microtubule and chromosome structure and decreased embryo apoptosis | [38] |
LC on oocyte maturation and parthenogenetic embryos in pigs | 0.25, 0.5, 1.0 and 2.0 mg/mL of LC in IVM medium | • Porcine ovaries were collected from prepubertal gilts and matured in medium containing various concentrations of LC. • LC was added in IVC medium to examine developmental competence of parthenogenic embryos | LC addition during IVM improved developmental potential of oocytes, and also quality of parthenogenic embryos by improving nuclear maturation and preventing OS and apoptosis | [14] |
LC on lipid metabolism and in vitro maturation of porcine oocyte | 0.3 to 10 mg/mL of LC | Ovaries from prepubertal cross-bred gilts were collected and IVF and IVC was performed in media containing LC | Enhanced mitochondrial functions, lipid metabolism for nuclear and cytoplasmic maturation of porcine oocytes | [54] |
LC on oocyte maturation and embryo development | 10 mM of LC in IVM medium | • Porcine ovaries were collected from 6 to 7 months old prepubertal gilts • Comparison of GSH, ROS levels and developmental gene expression in LC supplemented and non-supplemented group | • Reduced OS with increased GSH synthesis in LC supplemented group • Increased expression of developmental genes | [23] |
LC on maturation rate of buffalo embryos | 0.3, 0.6 and 1.2Â mM/mL of LC | Oocytes were collected from Swamp buffalo and treated with various concentrations of LC | Significantly higher metaphase II oocytes than control group with faster maturation rate | [66] |
LC on bovine embryo development and their cryotolerance | 1.1518 mM and 3.030 mM of LC | • Oocytes were collected from bovine ovaries • IVF and IVC was performed in media containing LC | Improved cryotolerance, lipid metabolism in embryos | [68] |
LC on vitrification of mouse germinal vesicle stage-oocytes and their in vitro maturation | 3.72 mM (0.6 mg/mL) of LC in IVM medium | • B6.DBA cross-bed mice were superovulated and oocytes were collected • Oocytes grown in LC-supplemented IVM medium | Increased number of metaphase II oocytes and improved mitochondrial distribution in oocytes | [55] |
ALC on lamb oocyte blastocyst rate, mitochondrial DNA copy number | 2 mM of ALC in IVM medium | Prepubertal lamb oocytes were collected and matured in medium containing LC | Increased cytoplasmic volume of oocyte with more lipid droplets, but no alteration in mitochondrial volume, number or DNA copy number | [22] |
LC on maturation of mouse embryos | 0.3 and 0.6 mg/mL of LC | • Immature oocytes were collected from NMRI mice ovaries and treated with LC • Cleavage rate, BDR and GSH were evaluated | Improved implantation developmental competence and nuclear maturation of oocytes and increased GSH | [64] |
LC on bovine blastocyst development | 0.1, 0.5 and 1.0 mg/mL of LC in IVM medium | • Oocytes were collected from bovine ovaries and matured in medium containing LC • These oocytes were then subjected to IVF with fresh semen • Number of embryos and total cell count was performed | Improved developmental potential: increased number of oocytes and embryos with higher total cell count | [70] |
LC in OS and antioxidant profile in sheep embryos produces in vitro | 2.5, 5, 7.5 and 10 mM of LC in maturation medium | Oocytes were collected from sheep ovaries and matured in medium containing LC. These oocytes were then subjected to IVF with fresh semen. | • Oocyte maturation, embryo development increased • LC reduced OS and ROS production, increased antioxidant enzyme activities | [56] |
LC on in vitro maturation of ZP and development of mouse embryos | 0.5, 1, 2 and 4 mg/ml of LC | • Mice were superovulated and mating was carried out with males • 2-cell embryos were flushed from oviduct and cultured in LC containing medium • BDR, ZP thickness were measured | Increased number of blastocyst cells, ZP thickness and improved antioxidant activity | [71] |