Fig. 3From: Live birth after Laser Assisted Viability Assessment (LAVA) to detect pentoxifylline resistant ejaculated immotile spermatozoa during ICSI in a couple with male Kartagener’s syndromeEosin-nigrosin staining for viability assessment. Eosin is used to mark dead cells which uptake the stain through damaged, porous membranes and appear red or pinkish (arrowhead), where live spermatozoa that hinder eosin to penetrate head region because of their intact membrane, appear white (arrow). Scale bar indicates 50 μmBack to article page