Fig. 2

Effects of AKT inhibitors III and IV treatment on bovine early embryonic development. Presumptive zygotes were cultured in the presence of 0, 25, 50 or 75 μM AKT inhibitor III or 0, 1.5, 2.5 or 3.5 μM AKT inhibitor IV until 72 hpi, then washed and cultured in fresh culture media lacking inhibitors until d 7 (n = 4 replicates/inhibitor, n = 25–30 embryos/treatment). Effects of each AKT inhibitor treatment on (a, e) early cleavage, (b, f) total cleavage, (c, g) development to 8- to 16-cell stage and (d, h) d7 blastocyst rates were determined. Data are expressed as mean ± SEM. Values with different superscripts among treatments indicate significant differences (P < 0.05)