Table 1 Primary and secondary antibodies used for Western blotting
Primary Antibody | Dilution | Catalogue Number | Company | Secondary Antibody | Dilution | Catalogue Number | Company |
|---|---|---|---|---|---|---|---|
Ang-(1–7) | 1:200 | PAS085Ra01 | Cloud-Clone Corporation | Anti-rabbit from donkey | 1:10,000 | NA934 | Amersham |
MME | 1:250 | Ab126593 | Abcam | Anti-rabbit from donkey | 1:10,000 | NA934 | Amersham |
ACE2 | 1:250 | Sc-20,998 | Santa Cruz | Anti-rabbit from goat | 1:5000 | Sc-2004 | Santa Cruz |
Mas1 | 1:100 | Sc-390,453 | Santa Cruz | Anti-mouse from sheep | 1:20,000 | NA931 | Amersham |
B-actin | 1:5000–1:20,000 | MAB1501R | Millipore corporation | Anti-mouse from sheep | 1:20,000 | NA931 | Amersham |