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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling

Fig. 4

Immunohistochemical analysis of ovarian tumor markers. a-j Expression of granulosa cell and germ cell markers in 8-week-old control and TGFBR1-CAG9Cre ovaries. Representative images from immunohistochemical analysis of FOXL2 (a and b), INHA (c and d), FOXO1 (e and f), AMH (g and h), and DDX4 (i and j) are shown. k and l Negative controls using isotype-matched rabbit and goat IgGs. Experiment was performed using ABC method, and signals were developed using NovaRED Peroxidase Substrate Kit. Sections were counterstained with hematoxylin. Five independent samples per group were utilized in this analysis. Scale bar is representatively depicted in (a) and equals 50 μm (a-l)

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