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Table 1 Gene-specific primers used in SSH and semiquantitative RT-PCR analysis

From: Gene expression profiling of upregulated mRNAs in granulosa cells of bovine ovulatory follicles following stimulation with hCG

Gene

 

Primer Sequence (5′-3′)a

GenBank accession number

ADAMTS1

Fwd

CGATAAATGTGGCATCTGTGGAG

NM_001101080

 

Rv

AGCCCACACGACTTGGAACACTC

 

ADAMTS9

Fwd

GGAAATCCGTATTGGGAATGCTG

NM_001206573

 

Rv

CATCTAGCCTGCTGTACTTGGC

 

ARAF

Fwd

TCTAACAACATCTTCCTGCACGAG

NM_001014964

 

Rv

GGCAACTCATCAGCCTGGGTAC

 

CAPN2

Fwd

TGAGGGCTTCGAGGACTTCACTG

NM_001103086

 

Rv

GCTGTCACTGGTGAGTGTGTCAG

 

CRISPLD2

Fwd

CTATGGGATCCTGGACGACAGG

AY369781

 

Rv

CCTGCAAGTTCACTGCCTGACG

 

CYP19A1

Fwd

GTCCGAAGTTGTGCCTATTGCCAGC

NM_174305

 

Rv

CCTCCAGCCTGTCCAGATGCTTGG

 

FKBP4

Fwd

TCCATTCCTCGTGAATGAATGTCC

NM_001034322

 

Rv

TGAACCTTCAGCAACGTGCAGAG

 

FKBP5

Fwd

ACTGGAGCAGGCTGCCATTGTC

XM_615814

 

Rv

CTTGAACATGTTGGCATAGATTCTG

 

GAPDH

Fwd

TGTTCCAGTATGATTCCACCCACG

NM_001034034

 

Rv

CTGTTGAAGTCGCAGGAGACAACC

 

KITLG1

Fwd

AGTAACCGTGTGACTGATGATGTG

NM_174375

 

Rv

AGAATGCTGGCAATGCTACGGCTG

 

KITLG2

Fwd

AGTAACCGTGTGACTGATGATGTG

NM_174375

 

Rv

CTAAGGGAGCTGGCTGCAACAG

 

L3MBTL3

Fwd

GTTATTACAGATGAGAGTGAGATGG

AY437805

 

Rv

ACTTAAAGGACAGAAAGACGGC

 

L3MBTL3

Fwd

CTTACCTGGATGTGAAGAACATGG

AY437805

(3’end of ORF)

Rv

CTGCAAGGTCTAAGACAGAGCTC

 

MMP1

Fwd

CTTGGACTTGCTCATTCTACTGAC

NM_174112

 

Rv

GGCATCGATGCTCTTCACCGTTC

 

MRO

Fwd

GGTGAGTTCTGAAGTCATCCATG

BC146146

 

Rv

CCTGGTGTGCCGACCACCTTC

 

NUDT10

Fwd

GACAGGTGAGCTCTTTCACACTC

NM_001035488

 

Rv

GGAGTTATGTCTAGAGGCACAGTC

 

NUDT11

Fwd

GAACAGCAAAGATGTCCAGGATTG

NM_001101296

 

Rv

CACACACATGGTGCCTGGAGAG

 

P4HA3

Fwd

TTGGCAAGGTGGCCTATGACATG

NM_001001598

 

Rv

CTGGAGCAGCAGGTAAGGACTG

 

POSTN

Fwd

CCAAAGCCCACTGCCAGTTCTC

AY445072

 

Rv

GAAAGCCACTTTGATGTGAAGAATGAG

 

PSAP

Fwd

CTTCCTCCTGTACCCTCAGGAC

NM_174161

 

Rv

GGGATGACGTTCTCTGAGACCAC

 

PTGS2

Fwd

GCATTCTTTGCCCAGCACTTCACCC

NM_174445

 

Rv

CTATCAGGATTAGCCTGCTTGTCTGG

 

RBP1

Fwd

CGGTCGACTTTACCGGGTACTG

NM_001025343

 

Rv

GTCATGTCACTCATTCCTAGAGAC

 

SDC4

Fwd

TCCGAGAAACTGAGGTCATCGAC

XM_584869

 

Rv

GGTACACCAGCAGCAGCACGAG

 

TIMP1

Fwd

CGTCATCAGGGCCAAGTTCGTG

NM_174471

 

Rv

GCAAGGACTGCCAGGTGCACAG

 

TIMP2

Fwd

TGCAATGCAGACATAGTGATCAGG

NM_174472

 

Rv

CGCTTCTCTTGATGCAGGCGAAG

 

TNC

Fwd

CTATGTGCCCATTGCAGGAGGTG

NM_001078026

 

Rv

AACTTGGTGGTGATGGTTGAGCTC

 

USP53

Fwd

GAGAGCATACCCACCAGTCAGATG

BC149007

 

Rv

GTTACTTTGACATCAGGAGTAGAGTC

 
  1. Fwd foward primer, Rv Reverse primer
  2. aAll primers were designed and validated by the authors