Fig. 4

Nuclear morphology of the spermatogenic process in different cell types along the culture progression. (a) Pachytene cell at culture day 10 of a 6.5 dpp explant. (b) Round spermatid at culture day 13 of a 6.5 dpp explant. (c) Sperm head at the elongation phase after 41 culture days of a 6.5 dpp explant. (d) Type A spermatogonium at culture day 4 of 6.5 dpp explant. (e) Type B spermatogonium at culture day 6 of 6.5 dpp explant. (f) Spermatogonial mitosis at culture day 6 of a 6.5 dpp explant. (g) Round spermatids at culture day 11 of a 10.5 dpp explant. (h) Elongated spermatid at culture day 22 of a 10.5 dpp explant. (i) Sperm heads at culture day 25 of a 10.5 dpp explant. DAPI stained. Scale bars represent = 5 μm