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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Chromatin status and transcription factor binding to gonadotropin promoters in gonadotrope cell lines

Fig. 4

Pituitary transcription factor accessibility in developing gonadotrope cell lines. a Expression levels of transcription factors in NIH3T3, αT1–1, αT3–1, LβT2, and TαT1 cell lines (n = 2). ChIP assays in αT1–1, αT3–1, and LβT2 cells were performed using antibodies specific to the transcription factors (b) LHX3 and (c) PITX1. Immunoprecipitated chromatin was subjected to qRT-PCR. Data are presented as fold enrichment relative to IgG, with values normalized to Gnrh1 and expressed as mean ± SEM. Statistical analysis by one-way ANOVA followed by a Tukey post-hoc, *p < 0.05, **p < 0.01, as indicated by bar. n.s.: non-significant. d LβT2 cells were treated with GnRH and/or activin (n = 3), before DNA from nuclei were treated with increasing concentrations of DNaseI and analyzed by qRT-PCR with primers specific to Lhb. Amplicon quantities were normalized to untreated samples (Vehicle), and data presented as the mean fraction of DNA remaining relative to vehicle

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