Fig. 4

Heat maps showing methylation distribution and CpG density distribution. Heat maps of (a) Prepubertal and (b) pubertal samples. n refers to the number of analyzed CpGs (per-strand depth ≥ 4) within that feature. CpG density (x-axis) is defined as the number of CpG dinucleotides within the 200 bp window. Methylation level (y-axis) is defined as average methylation level of cytosines in CpGs. The thin black lines within each heat map denote the median methylation level of CpGs at the given local density. The red gradient indicates abundance of CpGs that fall into bins of given methylation levels and CpG densities. The blue bar charts above each heat map show the distribution of CpG densities, projected onto the x-axis of the heat maps. The green bar charts to the right of the heat maps show the distribution of methylation levels, projected onto the y-axis of the heat maps