Table 1 Primers used for genotyping
Polymorphism | Primers | Annealing temp (°C) | Restriction enzyme | Band sizes (bp) |
|---|---|---|---|---|
TNF -238G > A | F: 5′-AAA CAG ACC ACA GAC CTG GTC-3′ | 59 | BamHI | G: 130, 20 |
R: 5′-CTC ACA CTC CCC ATC CTC CCG GAT C-3′ | A: 150 | |||
TNF -308G > A | F: 5′-GAG GCA ATA GGT TTT GAG GGC CAT-3′ | 58 | NcoI | G: 87, 20 |
R: 5′-GGG ACA CAC AAG CAT CAA G-3’ | A: 107 | |||
IL1B -511 T > C | F: 5′-TGG CAT TGA TCT GGT TCA TC-3’ | 58 | AvaI | T: 305 |
R: 5′-GTT TAG GAA TCT TCC CAC TT-3’ | C: 190, 115 | |||
IL1B 3954C > T | F: 5′-GTT GTC ATC AGA CTT TGA CC-3’ | 54 | TaqI | T: 250 |
R: 5′-TTC AGT TCA TAT GGA CCA GA-3’ | C: 136, 114 | |||
IL6 -174G > C | F: 5′-GGA GTC ACA CAC TCC ACC T-3’ | 59 | SfaNI | G: 532 |
R: 5′-GTG GGG CTG ATT GGA AAC C-3’ | C: 474, 58 | |||
IL6 -634C > G | F: 5′-GAG AGG CCT TGA AGT AAC TG-3’ | 58 | BsrBI | C: 180 |
R: 5′-AAC CAA AGA TGT TCT GAA CTG A-3’ | G: 120, 60 | |||
IL10 -1082A > G | F: 5′-GTA AGC TTC TGT GGC TGG AGT-3’ | 60 | MnlI | A: 130 |
R: 5′-TTT CCA GAT ATC TGA AGA AGT CCT G-3’ | G: 100, 30 | |||
IFNG 874A > T | F: 5′-TCA ACA AAG CTG ATA CTC CA-3’ | 61 | – | 261 |
R1: 5′-TTC TTA CAA CAC AAA ATC AAA TCA-3’ | ||||
R2: 5′-TTC TTA CAA CAC AAA ATC AAA TCT-3’ |