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Table 1 Primers used for genotyping

From: Association between TNF, IL1B, IL6, IL10 and IFNG polymorphisms and recurrent miscarriage: a case control study

Polymorphism Primers Annealing temp (°C) Restriction enzyme Band sizes (bp)
TNF -238G > A F: 5′-AAA CAG ACC ACA GAC CTG GTC-3′ 59 BamHI G: 130, 20
R: 5′-CTC ACA CTC CCC ATC CTC CCG GAT C-3′   A: 150
TNF -308G > A F: 5′-GAG GCA ATA GGT TTT GAG GGC CAT-3′ 58 NcoI G: 87, 20
R: 5′-GGG ACA CAC AAG CAT CAA G-3’   A: 107
IL1B -511 T > C F: 5′-TGG CAT TGA TCT GGT TCA TC-3’ 58 AvaI T: 305
R: 5′-GTT TAG GAA TCT TCC CAC TT-3’   C: 190, 115
IL1B 3954C > T F: 5′-GTT GTC ATC AGA CTT TGA CC-3’ 54 TaqI T: 250
R: 5′-TTC AGT TCA TAT GGA CCA GA-3’   C: 136, 114
IL6 -174G > C F: 5′-GGA GTC ACA CAC TCC ACC T-3’ 59 SfaNI G: 532
R: 5′-GTG GGG CTG ATT GGA AAC C-3’   C: 474, 58
IL6 -634C > G F: 5′-GAG AGG CCT TGA AGT AAC TG-3’ 58 BsrBI C: 180
R: 5′-AAC CAA AGA TGT TCT GAA CTG A-3’   G: 120, 60
IL10 -1082A > G F: 5′-GTA AGC TTC TGT GGC TGG AGT-3’ 60 MnlI A: 130
R: 5′-TTT CCA GAT ATC TGA AGA AGT CCT G-3’   G: 100, 30
IFNG 874A > T F: 5′-TCA ACA AAG CTG ATA CTC CA-3’ 61 261
R1: 5′-TTC TTA CAA CAC AAA ATC AAA TCA-3’   
R2: 5′-TTC TTA CAA CAC AAA ATC AAA TCT-3’