Effect of day 3 embryo morphometrics and morphokinetics on survival and implantation after slow freezing-thawing and after vitrification-warming: a retrospective cohort study

Table 2 Morphometric parameters during fresh culture before slow freezing or vitrification for survived and not survived embryos in dataset 1. Differences between survived and not survived embryos were calculated for each parameter using linear regression (95% CI and p values)

Freezing method	Parameter	Survived Mean ± SD	Not survived Mean ± SD	Coefficient survived vs not survived	95% CI	P
Slow freezing	n	71^b	66^c
	TCV, μm ³	715,113 ± 86,190	719,250 ± 98,140	−1309	−30,401 to 27,783	0.9289
	N blastomeres	8.2 ± 1.9	8.1 ± 1.3	0.02	−0.55 to 0.59	0.9371
	% Fragmentation ^a	13.7 ± 10.2	15.0 ± 9.6	−0.88	−4.25 to 2.49	0.6040
	% Symmetry	73.5 ± 8.2	71.1 ± 9.2	2.32	−0.63 to 5.28	0.1218
Vitrification	n	104^d	14
	TCV, μm ³	727,513 ± 101,655	688,627 ± 112,499	60,008	7159 to 112,858	0.0267
	N blastomeres	8.2 ± 1.8	8.9 ± 2.4	−0.19	−1.24 to 0.86	0.7164
	% Fragmentation	14.0 ± 10.0	19.3 ± 10.8	−5.55	−11.3 to 0.20	0.0584
	% Symmetry	72.4 ± 8.6	76.8 ± 6.8	−4.93	−9.72 to −0.15	0.0435

Random intercept correction was used to account for clustering
Bonferroni-Holm method was used per freezing method to correct for multiple testing
^a1 embryo excluded because ≤ − 20% fragmentation
No morphometrics could be analyzed in compacted embryos
^b8/79 embryos were compacted at evaluation
^c6/72 embryos were compacted at evaluation
^d8/112 embryos were compacted at evaluation

ISSN: 1477-7827