Fig. 5

Effects of GSK461364 on spindle assembly and chromosome segregation during ATI stage in porcine oocytes. In the control group, most α-tubulin assembled a normal spindle (a-a2) and homologous chromosomes were successfully separated (a-a1); GSK461364 treatment had no effect on spindle assembly (a-a5), but led to severe defects of chromosome segregation (a-a7) in porcine oocytes. (b) There was no significant difference in spindle assembly between the control and treatment groups. (c) The percentage of Plk1-inhibited oocytes with mis-segregated chromosomes was increased compared to the control oocytes. Green, spindle; blue, chromosome. Scale bar, 20 μm. *** P < 0.001