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Table 1 A list of 219 identified high-confidence HFF proteins from women underwent successful IVF by LC MALDI TOF/TOF mass spectrometry (FDR < 0.01)

From: Proteomic analysis of human follicular fluid associated with successful in vitro fertilization

No SwissProt AC Name protein description Gene Name Molecular Weight experiment 1 experiment 2
Coverage(%) Matched Peptides number Coverage(%) Matched Peptides number
1 P43652 Afamin AFM 69,069 31.9 10 35.7 10
2 P02763 Alpha-1-acid glycoprotein 1 ORM1 23,512 40.8 17 40.8 15
3 P19652 Alpha-1-acid glycoprotein 2 ORM2 23,603 45.8 15 53.2 15
4 P01011 Alpha-1-antichymotrypsin SERPINA3 47,651 53 15 44.2 16
5 P01009 Alpha-1-antitrypsin SERPINA1 46,737 62.7 86 64.4 76
6 P04217 Alpha-1B-glycoprotein A1BG 54,254 39.8 17 48.5 19
7 P08697 Alpha-2-antiplasmin SERPINF2 54,566 29.1 9 47.1 11
8 P02765 Alpha-2-HS-glycoprotein AHSG 39,325 42.8 14 55.9 18
9 P01023 Alpha-2-macroglobulin A2M 163,291 46.8 47 47.4 46
10 P48728 Aminomethyltransferase, mitochondrial AMT 43,946 2.2 1 - -
11 P01019 Angiotensinogen AGT 53,154 37.7 14 25.8 11
12 C9JTQ0 Ankyrin repeat domain-containing protein 63 ANKRD63 39,620 15 1 - -
13 P01008 Antithrombin-III SERPINC1 52,602 61.9 21 54.7 24
14 P02647 Apolipoprotein A-I APOA1 30,778 73.8 67 82.4 69
15 P02652 Apolipoprotein A-II APOA2 11,175 70 9 64 9
16 P06727 Apolipoprotein A-IV APOA4 45,399 67.2 24 63.1 25
17 P02654 Apolipoprotein C-I APOC1 9332 26.5 3 37.4 3
18 P02655 Apolipoprotein C-II APOC2 11,284 39.6 2 50.5 3
19 P02656 Apolipoprotein C-III APOC3 10,852 34.3 2 51.5 6
20 P05090 Apolipoprotein D APOD 21,276 24.9 3 28.6 3
21 P02649 Apolipoprotein E APOE 36,154 43.2 6 43.5 4
22 Q13790 Apolipoprotein F APOF 35,399 - - 8 1
23 O95445 Apolipoprotein M APOM 21,253 26.6 2 30.3 2
24 Q9H2U1 ATP-dependent RNA helicase DHX36 DHX36 114,760 - - 17.9 1
25 O75882 Attractin ATRN 158,537 15 1 - -
26 P98160 Basement membrane-specific heparan sulfate proteoglycan core protein HSPG2 468,830 30.8 43 31 46
27 P02749 Beta-2-glycoprotein 1 APOH 38,298 51 15 41.5 16
28 Q96KN2 Beta-Ala-His dipeptidase CNDP1 56,706 18.9 1 - -
29 P43251 Biotinidase BTD 61,133 9.2 2 14.2 1
30 Q7L273 BTB/POZ domain-containing protein KCTD9 KCTD9 42,567 - - 30.1 1
31 P04003 C4b-binding protein alpha chain C4BPA 67,033 11.9 2 27 4
32 Q96IY4 Carboxypeptidase B2 CPB2 48,424 13 2 16.1 2
33 P22792 Carboxypeptidase N subunit 2 CPN2 60,557 - - 10.8 2
34 Q9ULM6 CCR4-NOT transcription complex subunit 6 CNOT6 63,307 - - 2.3 1
35 Q8N8E3 Centrosomal protein of 112 kDa CEP112 112,749 17.4 1 - -
36 Q5SW79 Centrosomal protein of 170 kDa CEP170 175,293 - - 5.9 1
37 P00450 Ceruloplasmin CP 122,205 59.6 47 58.1 58
38 O14647 Chromodomain-helicase-DNA-binding protein 2 CHD2 211,344 - - 12 1
39 P10909 Clusterin CLU 52,495 41.4 14 50.1 12
40 P00740 Coagulation factor IX F9 51,778 15.2 1 - -
41 P00742 Coagulation factor X F10 54,732 24.6 1 14.1 1
42 P00748 Coagulation factor XII F12 67,792 29.9 4 20.8 4
43 Q5TID7 Coiled-coil domain-containing protein 181 CCDC181 60,103 - - 7.9 1
44 P02746 Complement C1q subcomponent subunit B C1QB 26,722 20.2 1 18.6 1
45 Q9NZP8 Complement C1r subcomponent-like protein C1RL 53,498 8.6 1 6.2 1
46 P06681 Complement C2 C2 83,268 21.5 4 22.7 6
47 P01024 Complement C3 C3 187,148 67.1 121 74.1 119
48 P0C0L4 Complement C4-A C4A 192,785 46.6 53 54.8 66
49 P0C0L5 Complement C4-B C4B 192,751 46.3 52 53 66
50 P01031 Complement C5 C5 188,305 20.3 7 27.1 12
51 P13671 Complement component C6 C6 104,786 26 6 25.5 6
52 P10643 Complement component C7 C7 93,518 35.2 8 23.1 5
53 P07357 Complement component C8 alpha chain C8A 65,163 24.8 5 23.5 4
54 P07358 Complement component C8 beta chain C8B 67,047 37.1 4 37.2 6
55 P07360 Complement component C8 gamma chain C8G 22,277 48.5 7 48 5
56 P02748 Complement component C9 C9 63,173 36.5 8 35.8 10
57 P00751 Complement factor B CFB 85,533 41.4 20 51.4 25
58 P08603 Complement factor H CFH 139,096 55.4 43 56.9 45
59 Q03591 Complement factor H-related protein 1 CFHR1 37,651 33.9 2 27.3 5
60 P05156 Complement factor I CFI 65,750 31.1 7 31.7 5
61 P08185 Corticosteroid-binding globulin SERPINA6 45,141 19.5 3 17.3 2
62 Q9UBG0 C-type mannose receptor 2 MRC2 166,674 3.2 1 - -
63 P01034 Cystatin-C CST3 15,799 22.6 1 - -
64 P30876 DNA-directed RNA polymerase II subunit RPB2 POLR2B 133,897 - - 10.7 1
65 Q8NHS0 DnaJ homolog subfamily B member 8 DNAJB8 25,686 16.8 1 - -
66 Q96DT5 Dynein heavy chain 11, axonemal DNAH11 520,369 - - 9.8 1
67 Q9C0C9 E2 ubiquitin-conjugating enzyme UBE2O 141,293 - - 3.9 1
68 O95071 E3 ubiquitin-protein ligase UBR5 UBR5 309,352 7.6 1 - -
69 A4FU69 EF-hand calcium-binding domain-containing protein 5 EFCAB5 173,404 8.1 1 - -
70 Q16610 Extracellular matrix protein 1 ECM1 60,674 20.7 2 11.5 2
71 Q9UGM5 Fetuin-B FETUB 42,055 12.8 1 18.3 1
72 P02671 Fibrinogen alpha chain FGA 94,973 44.8 40 47.6 44
73 P02675 Fibrinogen beta chain FGB 55,928 72.1 53 68.6 42
74 P02679 Fibrinogen gamma chain FGG 51,512 69.1 36 68 34
75 P02751 Fibronectin FN1 262,625 30.3 33 31.2 34
76 Q08380 Galectin-3-binding protein LGALS3BP 65,331 22.9 1 28.7 4
77 P06396 Gelsolin GSN 85,698 43.9 16 43.6 20
78 P07093 Glia-derived nexin SERPINE2 44,002 34.7 4 28.6 3
79 P22352 Glutathione peroxidase 3 GPX3 25,552 16.4 2 27 1
80 Q7Z4J2 Glycosyltransferase 6 domain-containing protein 1 GLT6D1 36,274 2.6 1 - -
81 P0CG08 Golgi pH regulator B GPR89B 52,917 - - 7.7 1
82 P00738 Haptoglobin HP 45,205 61.1 26 58.6 23
83 P00739 Haptoglobin-related protein HPR 39,030 44.3 10 - -
84 Q9Y6N9 Harmonin USH1C 62,211 7.8 1 - -
85 P69905 Hemoglobin subunit alpha HBA1/HBA2 15,258 - - 28.2 1
86 P68871 Hemoglobin subunit beta HBB 15,998 43.5 2 52.4 1
87 P02790 Hemopexin HPX 51,676 55.8 44 76.4 50
88 P05546 Heparin cofactor 2 SERPIND1 57,071 21 6 34.9 6
89 Q04756 Hepatocyte growth factor activator HGFAC 70,682 5.3 1 - -
90 P04196 Histidine-rich glycoprotein HRG 59,578 33 15 37.9 18
91 O43365 Homeobox protein Hox-A3 HOXA3 46,369 6.5 1 - -
92 P78426 Homeobox protein Nkx-6.1 NKX6–1 37,849 16.4 1 - -
93 Q14520 Hyaluronan-binding protein 2 HABP2 62,672 15.4 2 11.8 3
94 P0DOX2 Immunoglobulin alpha-2 heavy chain N/A 48,935 39.1 14 40.9 12
95 P0DOX3 Immunoglobulin delta heavy chain N/A 56,224 19.9 1 23.4 1
96 P0DOX4 Immunoglobulin epsilon heavy chain N/A 60,323 8.4 2 15.7 2
97 P0DOX5 Immunoglobulin gamma-1 heavy chain N/A 49,330 70.6 144 71.9 123
98 P01876 Immunoglobulin heavy constant alpha 1 IGHA1 37,655 42.8 23 48.2 16
99 P01859 Immunoglobulin heavy constant gamma 2 IGHG2 35,901 74.9 104 69.9 92
100 P01860 Immunoglobulin heavy constant gamma 3 IGHG3 41,287 72.4 69 78.3 65
101 P01861 Immunoglobulin heavy constant gamma 4 IGHG4 35,941 79.8 101 68.8 85
102 P01871 Immunoglobulin heavy constant mu IGHM 49,440 33.1 10 34.7 12
103 A0A0C4DH31 Immunoglobulin heavy variable 1–18 IGHV1–18 12,820 53 7 48.7 9
104 P23083 Immunoglobulin heavy variable 1–2 IGHV1–2 13,085 47.9 6 - -
105 A0A0C4DH33 Immunoglobulin heavy variable 1–24 IGHV1–24 12,824 38.5 2 38.5 3
106 A0A0C4DH29 Immunoglobulin heavy variable 1–3 IGHV1–3 13,008 38.5 3 - -
107 A0A0A0MS14 Immunoglobulin heavy variable 1–45 IGHV1–45 13,508 9.4 2 - -
108 P01743 Immunoglobulin heavy variable 1–46 IGHV1–46 12,933 - - 32.5 5
109 P01742 Immunoglobulin heavy variable 1–69 IGHV1–69 12,659 - - 34.2 5
110 P01762 Immunoglobulin heavy variable 3–11 IGHV3–11 12,909 38.5 10 53.9 11
111 P01766 Immunoglobulin heavy variable 3–13 IGHV3–13 12,506 60.3 6 - -
112 A0A0B4J1V0 Immunoglobulin heavy variable 3–15 IGHV3–15 12,926 55.5 8 42.9 7
113 P01764 Immunoglobulin heavy variable 3–23 IGHV3–23 12,582 60.7 15 54.7 10
114 A0A0B4J1X8 Immunoglobulin heavy variable 3–43 IGHV3–43 13,077 - - 34.8 6
115 A0A0A0MS15 Immunoglobulin heavy variable 3–49 IGHV3–49 13,056 47.1 3 50.4 3
116 A0A075B6Q5 Immunoglobulin heavy variable 3–64 IGHV3–64 12,891 59.3 2 18.6 1
117 A0A0C4DH42 Immunoglobulin heavy variable 3–66 IGHV3–66 12,698 61.2 14 55.2 10
118 P01780 Immunoglobulin heavy variable 3–7 IGHV3–7 12,943 76.9 14 77.8 12
119 A0A0B4J1Y9 Immunoglobulin heavy variable 3–72 IGHV3–72 13,203 55.5 9 - -
120 A0A0B4J1V6 Immunoglobulin heavy variable 3–73 IGHV3–73 12,858 58 3 58 4
121 P01782 Immunoglobulin heavy variable 3–9 IGHV3–9 12,945 51.7 8 51.7 9
122 P06331 Immunoglobulin heavy variable 4–34 IGHV4–34 13,815 - - 38.2 4
123 P01824 Immunoglobulin heavy variable 4–39 IGHV4–39 13,917 19.2 4 - -
124 A0A0C4DH38 Immunoglobulin heavy variable 5–51 IGHV5–51 12,675 66.7 9 50.4 8
125 P01834 Immunoglobulin kappa constant IGKC 11,765 88.8 50 92.5 37
126 P0DOX7 Immunoglobulin kappa light chain N/A 23,379 61.2 52 62.6 39
127 P04430 Immunoglobulin kappa variable 1–16 IGKV1–16 12,618 - - 34.2 2
128 A0A075B6S5 Immunoglobulin kappa variable 1–27 IGKV1–27 12,712 47 8 65 8
129 P01594 Immunoglobulin kappa variable 1–33 IGKV1–33 12,848 49.6 5 42.7 4
130 P01602 Immunoglobulin kappa variable 1–5 IGKV1–5 12,782 30.8 3 30.8 6
131 A0A0C4DH72 Immunoglobulin kappa variable 1–6 IGKV1–6 12,697 47 4 47 5
132 A0A0C4DH69 Immunoglobulin kappa variable 1–9 IGKV1–9 12,715 74.4 5 44.4 5
133 P01611 Immunoglobulin kappa variable 1D-12 IGKV1D-12 12,620 44.4 5 49.6 7
134 A0A0B4J2D9 Immunoglobulin kappa variable 1D-13 IGKV1D-13 12,569 13.7 1 - -
135 A0A075B6S4 Immunoglobulin kappa variable 1D-17 IGKV1D-17 12,835 28.2 1 43.6 2
136 P04432 Immunoglobulin kappa variable 1D-39 IGKV1D-39 12,737 47 6 47.9 6
137 P06310 Immunoglobulin kappa variable 2–30 IGKV2–30 13,185 50 5 63.3 7
138 P01615 Immunoglobulin kappa variable 2D-28 IGKV2D-28 12,957 33.3 5 40.8 5
139 A0A075B6S2 Immunoglobulin kappa variable 2D-29 IGKV2D-29 13,143 - - 20.8 5
140 P01614 Immunoglobulin kappa variable 2D-40 IGKV2D-40 13,310 37.2 6 37.2 5
141 P04433 Immunoglobulin kappa variable 3–11 IGKV3–11 12,575 54.8 16 49.6 10
142 P01624 Immunoglobulin kappa variable 3–15 IGKV3–15 12,496 42.6 9 50.4 8
143 P01619 Immunoglobulin kappa variable 3–20 IGKV3–20 12,557 70.7 16 70.7 14
144 A0A087WSY6 Immunoglobulin kappa variable 3D-15 IGKV3D-15 12,534 42.6 10 56.5 8
145 A0A0C4DH25 Immunoglobulin kappa variable 3D-20 IGKV3D-20 12,515 64.7 10 64.7 8
146 P06312 Immunoglobulin kappa variable 4–1 IGKV4–1 13,380 34.7 10 40.5 6
147 A0M8Q6 Immunoglobulin lambda constant 7 IGLC7 11,254 54.7 13 53.8 10
148 A0A0B4J1U3 Immunoglobulin lambda variable 1–36 IGLV1–36 12,478 13.7 1 13.7 1
149 P01703 Immunoglobulin lambda variable 1–40 IGLV1–40 12,302 21.2 2 - -
150 P01700 Immunoglobulin lambda variable 1–47 IGLV1–47 12,284 54.7 4 39.3 3
151 P01706 Immunoglobulin lambda variable 2–11 IGLV2–11 12,644 22.7 3 - -
152 A0A075B6K4 Immunoglobulin lambda variable 3–10 IGLV3–10 12,441 40 4 40 3
153 P01714 Immunoglobulin lambda variable 3–19 IGLV3–19 12,042 50 2 42.9 1
154 P80748 Immunoglobulin lambda variable 3–21 IGLV3–21 12,446 35.9 3 - -
155 P01717 Immunoglobulin lambda variable 3–25 IGLV3–25 12,011 - - 43.8 3
156 P01721 Immunoglobulin lambda variable 6–57 IGLV6–57 12,566 20.5 2 - -
157 P0DOX8 Immunoglobulin lambda-1 light chain N/A 22,830 44.4 23 44.4 20
158 P15814 Immunoglobulin lambda-like polypeptide 1 IGLL1 22,963 23 5 23 5
159 P35858 Insulin-like growth factor-binding protein complex acid labile subunit IGFALS 66,035 23.1 4 27.4 6
160 P16144 Integrin beta-4 ITGB4 202,167 4.9 1 - -
161 P19827 Inter-alpha-trypsin inhibitor heavy chain H1 ITIH1 101,389 33.6 20 33.7 25
162 P19823 Inter-alpha-trypsin inhibitor heavy chain H2 ITIH2 106,463 35.9 18 42.6 20
163 Q06033 Inter-alpha-trypsin inhibitor heavy chain H3 ITIH3 99,849 5.2 1 15.5 1
164 Q14624 Inter-alpha-trypsin inhibitor heavy chain H4 ITIH4 103,357 38.4 23 47 26
165 Q15811 Intersectin-1 ITSN1 195,422 - - 9.9 1
166 P29622 Kallistatin SERPINA4 48,542 26.5 4 23 5
167 Q92764 Keratin, type I cuticular Ha5 KRT35 50,361 - - 16.7 1
168 P13645 Keratin, type I cytoskeletal 10 KRT10 58,827 5.8 1 - -
169 P04264 Keratin, type II cytoskeletal 1 KRT1 66,039 23.6 3 30 2
170 P01042 Kininogen-1 KNG1 71,957 53.7 25 41 23
171 P02750 Leucine-rich alpha-2-glycoprotein LRG1 38,178 21.6 4 27.1 5
172 P18428 Lipopolysaccharide-binding protein LBP 53,384 14.8 1 13.3 1
173 P51884 Lumican LUM 38,429 30.2 3 27.8 3
174 P14174 Macrophage migration inhibitory factor MIF 12,476 18.3 2 - -
175 P01033 Metalloproteinase inhibitor 1 TIMP1 23,171 18.8 2 34.8 2
176 Q7Z5P9 Mucin-19 MUC19 805,253 4.3 1 - -
177 P35579 Myosin-9 MYH9 226,532 - - 15.8 1
178 Q96PD5 N-acetylmuramoyl-L-alanine amidase PGLYRP2 62,217 26 7 29.3 6
179 A6NHN0 Otolin-1 OTOL1 49,422 15.3 1 - -
180 P04180 Phosphatidylcholine-sterol acyltransferase LCAT 49,578 15.5 2 - -
181 P36955 Pigment epithelium-derived factor SERPINF1 46,312 22.3 5 17.9 5
182 P03952 Plasma kallikrein KLKB1 71,370 23 6 26.5 6
183 P05155 Plasma protease C1 inhibitor SERPING1 55,154 34.8 9 33.2 16
184 P05154 Plasma serine protease inhibitor SERPINA5 45,675 13.6 3 - -
185 P00747 Plasminogen PLG 90,569 63 30 58.8 32
186 Q96GD3 Polycomb protein SCMH1 SCMH1 73,354 4.7 1 - -
187 Q8WUM4 Programmed cell death 6-interacting protein PDCD6IP 96,023 - - 14.1 1
188 P46013 Proliferation marker protein Ki-67 MKI67 358,694 11.9 1 21.8 1
189 P15309 Prostatic acid phosphatase ACPP 44,566 25.1 4 17.9 2
190 P02760 Protein AMBP AMBP 38,999 38.9 11 42.1 12
191 Q9UK55 Protein Z-dependent protease inhibitor SERPINA10 50,707 15.5 2 18.9 2
192 Q96PF1 Protein-glutamine gamma-glutamyltransferase Z TGM7 79,941 - - 7.5 1
193 P00734 Prothrombin F2 70,037 59.8 33 62.4 31
194 P02753 Retinol-binding protein 4 RBP4 23,010 40.3 11 55.7 13
195 O94885 SAM and SH3 domain-containing protein 1 SASH1 136,653 - - 10.3 1
196 P04279 Semenogelin-1 SEMG1 52,131 30.5 5 32.3 5
197 Q02383 Semenogelin-2 SEMG2 65,444 21 3 18 5
198 P57059 Serine/threonine-protein kinase SIK1 SIK1 84,902 - - 7.3 1
199 P02787 Serotransferrin TF 77,064 71.4 143 79.4 185
200 P02768 Serum albumin ALB 69,367 89.3 607 91.3 550
201 P35542 Serum amyloid A-4 protein SAA4 14,747 30 2 49.2 6
202 P02743 Serum amyloid P-component APCS 25,387 26.5 5 25.1 5
203 P27169 Serum paraoxonase/arylesterase 1 PON1 39,731 24.5 7 19.2 5
204 P04278 Sex hormone-binding globulin SHBG 43,779 18.7 4 21.9 3
205 P09486 SPARC SPARC 34,632 - - 5.3 1
206 Q6N022 Teneurin-4 TENM4 307,957 5.3 1 - -
207 P05452 Tetranectin CLEC3B 22,537 22.8 2 30.2 2
208 P05543 Thyroxine-binding globulin SERPINA7 46,325 14.5 1 23.6 2
209 Q8WZ42 Titin TTN 3,816,030 10.6 1 - -
210 P21675 Transcription initiation factor TFIID subunit 1 TAF1 212,677 - - 7 1
211 Q66K66 Transmembrane protein 198 TMEM198 39,475 2.5 2 2.5 1
212 P02766 Transthyretin TTR 15,887 69.4 12 69.4 19
213 P13611 Versican core protein VCAN 372,820 - - 5.2 2
214 P02774 Vitamin D-binding protein GC 52,964 63.9 29 60.3 28
215 P04070 Vitamin K-dependent protein C PROC 52,071 - - 2.2 1
216 P07225 Vitamin K-dependent protein S PROS1 75,123 12.6 2 - -
217 P04004 Vitronectin VTN 54,306 32.6 11 32.2 15
218 Q6PF04 Zinc finger protein 613 ZNF613 70,143 6.6 1 - -
219 P25311 Zinc-alpha-2-glycoprotein AZGP1 34,259 52.7 14 52 17