Table 1 A list of 219 identified high-confidence HFF proteins from women underwent successful IVF by LC MALDI TOF/TOF mass spectrometry (FDR < 0.01)

1

P43652

Afamin

AFM

69,069

31.9

10

35.7

10

2

P02763

Alpha-1-acid glycoprotein 1

ORM1

23,512

40.8

17

40.8

15

3

P19652

Alpha-1-acid glycoprotein 2

ORM2

23,603

45.8

15

53.2

15

4

P01011

Alpha-1-antichymotrypsin

SERPINA3

47,651

53

15

44.2

16

5

P01009

Alpha-1-antitrypsin

SERPINA1

46,737

62.7

86

64.4

76

6

P04217

Alpha-1B-glycoprotein

A1BG

54,254

39.8

17

48.5

19

7

P08697

Alpha-2-antiplasmin

SERPINF2

54,566

29.1

9

47.1

11

8

P02765

Alpha-2-HS-glycoprotein

AHSG

39,325

42.8

14

55.9

18

9

P01023

Alpha-2-macroglobulin

A2M

163,291

46.8

47

47.4

46

10

P48728

Aminomethyltransferase, mitochondrial

AMT

43,946

2.2

1

-

-

11

P01019

Angiotensinogen

AGT

53,154

37.7

14

25.8

11

12

C9JTQ0

Ankyrin repeat domain-containing protein 63

ANKRD63

39,620

15

1

-

-

13

P01008

Antithrombin-III

SERPINC1

52,602

61.9

21

54.7

24

14

P02647

Apolipoprotein A-I

APOA1

30,778

73.8

67

82.4

69

15

P02652

Apolipoprotein A-II

APOA2

11,175

70

9

64

9

16

P06727

Apolipoprotein A-IV

APOA4

45,399

67.2

24

63.1

25

17

P02654

Apolipoprotein C-I

APOC1

9332

26.5

3

37.4

3

18

P02655

Apolipoprotein C-II

APOC2

11,284

39.6

2

50.5

3

19

P02656

Apolipoprotein C-III

APOC3

10,852

34.3

2

51.5

6

20

P05090

Apolipoprotein D

APOD

21,276

24.9

3

28.6

3

21

P02649

Apolipoprotein E

APOE

36,154

43.2

6

43.5

4

22

Q13790

Apolipoprotein F

APOF

35,399

-

-

8

1

23

O95445

Apolipoprotein M

APOM

21,253

26.6

2

30.3

2

24

Q9H2U1

ATP-dependent RNA helicase DHX36

DHX36

114,760

-

-

17.9

1

25

O75882

Attractin

ATRN

158,537

15

1

-

-

26

P98160

Basement membrane-specific heparan sulfate proteoglycan core protein

HSPG2

468,830

30.8

43

31

46

27

P02749

Beta-2-glycoprotein 1

APOH

38,298

51

15

41.5

16

28

Q96KN2

Beta-Ala-His dipeptidase

CNDP1

56,706

18.9

1

-

-

29

P43251

Biotinidase

BTD

61,133

9.2

2

14.2

1

30

Q7L273

BTB/POZ domain-containing protein KCTD9

KCTD9

42,567

-

-

30.1

1

31

P04003

C4b-binding protein alpha chain

C4BPA

67,033

11.9

2

27

4

32

Q96IY4

Carboxypeptidase B2

CPB2

48,424

13

2

16.1

2

33

P22792

Carboxypeptidase N subunit 2

CPN2

60,557

-

-

10.8

2

34

Q9ULM6

CCR4-NOT transcription complex subunit 6

CNOT6

63,307

-

-

2.3

1

35

Q8N8E3

Centrosomal protein of 112 kDa

CEP112

112,749

17.4

1

-

-

36

Q5SW79

Centrosomal protein of 170 kDa

CEP170

175,293

-

-

5.9

1

37

P00450

Ceruloplasmin

CP

122,205

59.6

47

58.1

58

38

O14647

Chromodomain-helicase-DNA-binding protein 2

CHD2

211,344

-

-

12

1

39

P10909

Clusterin

CLU

52,495

41.4

14

50.1

12

40

P00740

Coagulation factor IX

F9

51,778

15.2

1

-

-

41

P00742

Coagulation factor X

F10

54,732

24.6

1

14.1

1

42

P00748

Coagulation factor XII

F12

67,792

29.9

4

20.8

4

43

Q5TID7

Coiled-coil domain-containing protein 181

CCDC181

60,103

-

-

7.9

1

44

P02746

Complement C1q subcomponent subunit B

C1QB

26,722

20.2

1

18.6

1

45

Q9NZP8

Complement C1r subcomponent-like protein

C1RL

53,498

8.6

1

6.2

1

46

P06681

Complement C2

C2

83,268

21.5

4

22.7

6

47

P01024

Complement C3

C3

187,148

67.1

121

74.1

119

48

P0C0L4

Complement C4-A

C4A

192,785

46.6

53

54.8

66

49

P0C0L5

Complement C4-B

C4B

192,751

46.3

52

53

66

50

P01031

Complement C5

C5

188,305

20.3

7

27.1

12

51

P13671

Complement component C6

C6

104,786

26

6

25.5

6

52

P10643

Complement component C7

C7

93,518

35.2

8

23.1

5

53

P07357

Complement component C8 alpha chain

C8A

65,163

24.8

5

23.5

4

54

P07358

Complement component C8 beta chain

C8B

67,047

37.1

4

37.2

6

55

P07360

Complement component C8 gamma chain

C8G

22,277

48.5

7

48

5

56

P02748

Complement component C9

C9

63,173

36.5

8

35.8

10

57

P00751

Complement factor B

CFB

85,533

41.4

20

51.4

25

58

P08603

Complement factor H

CFH

139,096

55.4

43

56.9

45

59

Q03591

Complement factor H-related protein 1

CFHR1

37,651

33.9

2

27.3

5

60

P05156

Complement factor I

CFI

65,750

31.1

7

31.7

5

61

P08185

Corticosteroid-binding globulin

SERPINA6

45,141

19.5

3

17.3

2

62

Q9UBG0

C-type mannose receptor 2

MRC2

166,674

3.2

1

-

-

63

P01034

Cystatin-C

CST3

15,799

22.6

1

-

-

64

P30876

DNA-directed RNA polymerase II subunit RPB2

POLR2B

133,897

-

-

10.7

1

65

Q8NHS0

DnaJ homolog subfamily B member 8

DNAJB8

25,686

16.8

1

-

-

66

Q96DT5

Dynein heavy chain 11, axonemal

DNAH11

520,369

-

-

9.8

1

67

Q9C0C9

E2 ubiquitin-conjugating enzyme

UBE2O

141,293

-

-

3.9

1

68

O95071

E3 ubiquitin-protein ligase UBR5

UBR5

309,352

7.6

1

-

-

69

A4FU69

EF-hand calcium-binding domain-containing protein 5

EFCAB5

173,404

8.1

1

-

-

70

Q16610

Extracellular matrix protein 1

ECM1

60,674

20.7

2

11.5

2

71

Q9UGM5

Fetuin-B

FETUB

42,055

12.8

1

18.3

1

72

P02671

Fibrinogen alpha chain

FGA

94,973

44.8

40

47.6

44

73

P02675

Fibrinogen beta chain

FGB

55,928

72.1

53

68.6

42

74

P02679

Fibrinogen gamma chain

FGG

51,512

69.1

36

68

34

75

P02751

Fibronectin

FN1

262,625

30.3

33

31.2

34

76

Q08380

Galectin-3-binding protein

LGALS3BP

65,331

22.9

1

28.7

4

77

P06396

Gelsolin

GSN

85,698

43.9

16

43.6

20

78

P07093

Glia-derived nexin

SERPINE2

44,002

34.7

4

28.6

3

79

P22352

Glutathione peroxidase 3

GPX3

25,552

16.4

2

27

1

80

Q7Z4J2

Glycosyltransferase 6 domain-containing protein 1

GLT6D1

36,274

2.6

1

-

-

81

P0CG08

Golgi pH regulator B

GPR89B

52,917

-

-

7.7

1

82

P00738

Haptoglobin

HP

45,205

61.1

26

58.6

23

83

P00739

Haptoglobin-related protein

HPR

39,030

44.3

10

-

-

84

Q9Y6N9

Harmonin

USH1C

62,211

7.8

1

-

-

85

P69905

Hemoglobin subunit alpha

HBA1/HBA2

15,258

-

-

28.2

1

86

P68871

Hemoglobin subunit beta

HBB

15,998

43.5

2

52.4

1

87

P02790

Hemopexin

HPX

51,676

55.8

44

76.4

50

88

P05546

Heparin cofactor 2

SERPIND1

57,071

21

6

34.9

6

89

Q04756

Hepatocyte growth factor activator

HGFAC

70,682

5.3

1

-

-

90

P04196

Histidine-rich glycoprotein

HRG

59,578

33

15

37.9

18

91

O43365

Homeobox protein Hox-A3

HOXA3

46,369

6.5

1

-

-

92

P78426

Homeobox protein Nkx-6.1

NKX6–1

37,849

16.4

1

-

-

93

Q14520

Hyaluronan-binding protein 2

HABP2

62,672

15.4

2

11.8

3

94

P0DOX2

Immunoglobulin alpha-2 heavy chain

N/A

48,935

39.1

14

40.9

12

95

P0DOX3

Immunoglobulin delta heavy chain

N/A

56,224

19.9

1

23.4

1

96

P0DOX4

Immunoglobulin epsilon heavy chain

N/A

60,323

8.4

2

15.7

2

97

P0DOX5

Immunoglobulin gamma-1 heavy chain

N/A

49,330

70.6

144

71.9

123

98

P01876

Immunoglobulin heavy constant alpha 1

IGHA1

37,655

42.8

23

48.2

16

99

P01859

Immunoglobulin heavy constant gamma 2

IGHG2

35,901

74.9

104

69.9

92

100

P01860

Immunoglobulin heavy constant gamma 3

IGHG3

41,287

72.4

69

78.3

65

101

P01861

Immunoglobulin heavy constant gamma 4

IGHG4

35,941

79.8

101

68.8

85

102

P01871

Immunoglobulin heavy constant mu

IGHM

49,440

33.1

10

34.7

12

103

A0A0C4DH31

Immunoglobulin heavy variable 1–18

IGHV1–18

12,820

53

7

48.7

9

104

P23083

Immunoglobulin heavy variable 1–2

IGHV1–2

13,085

47.9

6

-

-

105

A0A0C4DH33

Immunoglobulin heavy variable 1–24

IGHV1–24

12,824

38.5

2

38.5

3

106

A0A0C4DH29

Immunoglobulin heavy variable 1–3

IGHV1–3

13,008

38.5

3

-

-

107

A0A0A0MS14

Immunoglobulin heavy variable 1–45

IGHV1–45

13,508

9.4

2

-

-

108

P01743

Immunoglobulin heavy variable 1–46

IGHV1–46

12,933

-

-

32.5

5

109

P01742

Immunoglobulin heavy variable 1–69

IGHV1–69

12,659

-

-

34.2

5

110

P01762

Immunoglobulin heavy variable 3–11

IGHV3–11

12,909

38.5

10

53.9

11

111

P01766

Immunoglobulin heavy variable 3–13

IGHV3–13

12,506

60.3

6

-

-

112

A0A0B4J1V0

Immunoglobulin heavy variable 3–15

IGHV3–15

12,926

55.5

8

42.9

7

113

P01764

Immunoglobulin heavy variable 3–23

IGHV3–23

12,582

60.7

15

54.7

10

114

A0A0B4J1X8

Immunoglobulin heavy variable 3–43

IGHV3–43

13,077

-

-

34.8

6

115

A0A0A0MS15

Immunoglobulin heavy variable 3–49

IGHV3–49

13,056

47.1

3

50.4

3

116

A0A075B6Q5

Immunoglobulin heavy variable 3–64

IGHV3–64

12,891

59.3

2

18.6

1

117

A0A0C4DH42

Immunoglobulin heavy variable 3–66

IGHV3–66

12,698

61.2

14

55.2

10

118

P01780

Immunoglobulin heavy variable 3–7

IGHV3–7

12,943

76.9

14

77.8

12

119

A0A0B4J1Y9

Immunoglobulin heavy variable 3–72

IGHV3–72

13,203

55.5

9

-

-

120

A0A0B4J1V6

Immunoglobulin heavy variable 3–73

IGHV3–73

12,858

58

3

58

4

121

P01782

Immunoglobulin heavy variable 3–9

IGHV3–9

12,945

51.7

8

51.7

9

122

P06331

Immunoglobulin heavy variable 4–34

IGHV4–34

13,815

-

-

38.2

4

123

P01824

Immunoglobulin heavy variable 4–39

IGHV4–39

13,917

19.2

4

-

-

124

A0A0C4DH38

Immunoglobulin heavy variable 5–51

IGHV5–51

12,675

66.7

9

50.4

8

125

P01834

Immunoglobulin kappa constant

IGKC

11,765

88.8

50

92.5

37

126

P0DOX7

Immunoglobulin kappa light chain

N/A

23,379

61.2

52

62.6

39

127

P04430

Immunoglobulin kappa variable 1–16

IGKV1–16

12,618

-

-

34.2

2

128

A0A075B6S5

Immunoglobulin kappa variable 1–27

IGKV1–27

12,712

47

8

65

8

129

P01594

Immunoglobulin kappa variable 1–33

IGKV1–33

12,848

49.6

5

42.7

4

130

P01602

Immunoglobulin kappa variable 1–5

IGKV1–5

12,782

30.8

3

30.8

6

131

A0A0C4DH72

Immunoglobulin kappa variable 1–6

IGKV1–6

12,697

47

4

47

5

132

A0A0C4DH69

Immunoglobulin kappa variable 1–9

IGKV1–9

12,715

74.4

5

44.4

5

133

P01611

Immunoglobulin kappa variable 1D-12

IGKV1D-12

12,620

44.4

5

49.6

7

134

A0A0B4J2D9

Immunoglobulin kappa variable 1D-13

IGKV1D-13

12,569

13.7

1

-

-

135

A0A075B6S4

Immunoglobulin kappa variable 1D-17

IGKV1D-17

12,835

28.2

1

43.6

2

136

P04432

Immunoglobulin kappa variable 1D-39

IGKV1D-39

12,737

47

6

47.9

6

137

P06310

Immunoglobulin kappa variable 2–30

IGKV2–30

13,185

50

5

63.3

7

138

P01615

Immunoglobulin kappa variable 2D-28

IGKV2D-28

12,957

33.3

5

40.8

5

139

A0A075B6S2

Immunoglobulin kappa variable 2D-29

IGKV2D-29

13,143

-

-

20.8

5

140

P01614

Immunoglobulin kappa variable 2D-40

IGKV2D-40

13,310

37.2

6

37.2

5

141

P04433

Immunoglobulin kappa variable 3–11

IGKV3–11

12,575

54.8

16

49.6

10

142

P01624

Immunoglobulin kappa variable 3–15

IGKV3–15

12,496

42.6

9

50.4

8

143

P01619

Immunoglobulin kappa variable 3–20

IGKV3–20

12,557

70.7

16

70.7

14

144

A0A087WSY6

Immunoglobulin kappa variable 3D-15

IGKV3D-15

12,534

42.6

10

56.5

8

145

A0A0C4DH25

Immunoglobulin kappa variable 3D-20

IGKV3D-20

12,515

64.7

10

64.7

8

146

P06312

Immunoglobulin kappa variable 4–1

IGKV4–1

13,380

34.7

10

40.5

6

147

A0M8Q6

Immunoglobulin lambda constant 7

IGLC7

11,254

54.7

13

53.8

10

148

A0A0B4J1U3

Immunoglobulin lambda variable 1–36

IGLV1–36

12,478

13.7

1

13.7

1

149

P01703

Immunoglobulin lambda variable 1–40

IGLV1–40

12,302

21.2

2

-

-

150

P01700

Immunoglobulin lambda variable 1–47

IGLV1–47

12,284

54.7

4

39.3

3

151

P01706

Immunoglobulin lambda variable 2–11

IGLV2–11

12,644

22.7

3

-

-

152

A0A075B6K4

Immunoglobulin lambda variable 3–10

IGLV3–10

12,441

40

4

40

3

153

P01714

Immunoglobulin lambda variable 3–19

IGLV3–19

12,042

50

2

42.9

1

154

P80748

Immunoglobulin lambda variable 3–21

IGLV3–21

12,446

35.9

3

-

-

155

P01717

Immunoglobulin lambda variable 3–25

IGLV3–25

12,011

-

-

43.8

3

156

P01721

Immunoglobulin lambda variable 6–57

IGLV6–57

12,566

20.5

2

-

-

157

P0DOX8

Immunoglobulin lambda-1 light chain

N/A

22,830

44.4

23

44.4

20

158

P15814

Immunoglobulin lambda-like polypeptide 1

IGLL1

22,963

23

5

23

5

159

P35858

Insulin-like growth factor-binding protein complex acid labile subunit

IGFALS

66,035

23.1

4

27.4

6

160

P16144

Integrin beta-4

ITGB4

202,167

4.9

1

-

-

161

P19827

Inter-alpha-trypsin inhibitor heavy chain H1

ITIH1

101,389

33.6

20

33.7

25

162

P19823

Inter-alpha-trypsin inhibitor heavy chain H2

ITIH2

106,463

35.9

18

42.6

20

163

Q06033

Inter-alpha-trypsin inhibitor heavy chain H3

ITIH3

99,849

5.2

1

15.5

1

164

Q14624

Inter-alpha-trypsin inhibitor heavy chain H4

ITIH4

103,357

38.4

23

47

26

165

Q15811

Intersectin-1

ITSN1

195,422

-

-

9.9

1

166

P29622

Kallistatin

SERPINA4

48,542

26.5

4

23

5

167

Q92764

Keratin, type I cuticular Ha5

KRT35

50,361

-

-

16.7

1

168

P13645

Keratin, type I cytoskeletal 10

KRT10

58,827

5.8

1

-

-

169

P04264

Keratin, type II cytoskeletal 1

KRT1

66,039

23.6

3

30

2

170

P01042

Kininogen-1

KNG1

71,957

53.7

25

41

23

171

P02750

Leucine-rich alpha-2-glycoprotein

LRG1

38,178

21.6

4

27.1

5

172

P18428

Lipopolysaccharide-binding protein

LBP

53,384

14.8

1

13.3

1

173

P51884

Lumican

LUM

38,429

30.2

3

27.8

3

174

P14174

Macrophage migration inhibitory factor

MIF

12,476

18.3

2

-

-

175

P01033

Metalloproteinase inhibitor 1

TIMP1

23,171

18.8

2

34.8

2

176

Q7Z5P9

Mucin-19

MUC19

805,253

4.3

1

-

-

177

P35579

Myosin-9

MYH9

226,532

-

-

15.8

1

178

Q96PD5

N-acetylmuramoyl-L-alanine amidase

PGLYRP2

62,217

26

7

29.3

6

179

A6NHN0

Otolin-1

OTOL1

49,422

15.3

1

-

-

180

P04180

Phosphatidylcholine-sterol acyltransferase

LCAT

49,578

15.5

2

-

-

181

P36955

Pigment epithelium-derived factor

SERPINF1

46,312

22.3

5

17.9

5

182

P03952

Plasma kallikrein

KLKB1

71,370

23

6

26.5

6

183

P05155

Plasma protease C1 inhibitor

SERPING1

55,154

34.8

9

33.2

16

184

P05154

Plasma serine protease inhibitor

SERPINA5

45,675

13.6

3

-

-

185

P00747

Plasminogen

PLG

90,569

63

30

58.8

32

186

Q96GD3

Polycomb protein SCMH1

SCMH1

73,354

4.7

1

-

-

187

Q8WUM4

Programmed cell death 6-interacting protein

PDCD6IP

96,023

-

-

14.1

1

188

P46013

Proliferation marker protein Ki-67

MKI67

358,694

11.9

1

21.8

1

189

P15309

Prostatic acid phosphatase

ACPP

44,566

25.1

4

17.9

2

190

P02760

Protein AMBP

AMBP

38,999

38.9

11

42.1

12

191

Q9UK55

Protein Z-dependent protease inhibitor

SERPINA10

50,707

15.5

2

18.9

2

192

Q96PF1

Protein-glutamine gamma-glutamyltransferase Z

TGM7

79,941

-

-

7.5

1

193

P00734

Prothrombin

F2

70,037

59.8

33

62.4

31

194

P02753

Retinol-binding protein 4

RBP4

23,010

40.3

11

55.7

13

195

O94885

SAM and SH3 domain-containing protein 1

SASH1

136,653

-

-

10.3

1

196

P04279

Semenogelin-1

SEMG1

52,131

30.5

5

32.3

5

197

Q02383

Semenogelin-2

SEMG2

65,444

21

3

18

5

198

P57059

Serine/threonine-protein kinase SIK1

SIK1

84,902

-

-

7.3

1

199

P02787

Serotransferrin

TF

77,064

71.4

143

79.4

185

200

P02768

Serum albumin

ALB

69,367

89.3

607

91.3

550

201

P35542

Serum amyloid A-4 protein

SAA4

14,747

30

2

49.2

6

202

P02743

Serum amyloid P-component

APCS

25,387

26.5

5

25.1

5

203

P27169

Serum paraoxonase/arylesterase 1

PON1

39,731

24.5

7

19.2

5

204

P04278

Sex hormone-binding globulin

SHBG

43,779

18.7

4

21.9

3

205

P09486

SPARC

SPARC

34,632

-

-

5.3

1

206

Q6N022

Teneurin-4

TENM4

307,957

5.3

1

-

-

207

P05452

Tetranectin

CLEC3B

22,537

22.8

2

30.2

2

208

P05543

Thyroxine-binding globulin

SERPINA7

46,325

14.5

1

23.6

2

209

Q8WZ42

Titin

TTN

3,816,030

10.6

1

-

-

210

P21675

Transcription initiation factor TFIID subunit 1

TAF1

212,677

-

-

7

1

211

Q66K66

Transmembrane protein 198

TMEM198

39,475

2.5

2

2.5

1

212

P02766

Transthyretin

TTR

15,887

69.4

12

69.4

19

213

P13611

Versican core protein

VCAN

372,820

-

-

5.2

2

214

P02774

Vitamin D-binding protein

GC

52,964

63.9

29

60.3

28

215

P04070

Vitamin K-dependent protein C

PROC

52,071

-

-

2.2

1

216

P07225

Vitamin K-dependent protein S

PROS1

75,123

12.6

2

-

-

217

P04004

Vitronectin

VTN

54,306

32.6

11

32.2

15

218

Q6PF04

Zinc finger protein 613

ZNF613

70,143

6.6

1

-

-

219

P25311

Zinc-alpha-2-glycoprotein

AZGP1

34,259

52.7

14

52

17