Skip to main content
Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study

Fig. 5

Detection of TH2B protein using Western blotting and regression models showing variation in the intensity of TH2B in sperm as related to fertility scores in Holstein bulls. a MM stands for molecular markers; LF1–5 and HF1–5 refer to samples from low vs. high fertility bulls, respectively. The same amounts of nuclear proteins were loaded into each lane. As TH2B is a nuclear protein and the expression levels of our internal control (Lamin B1) were not stable, another protein band (~25 KDa) served as the internal control. b The regression line was determined using the model predicted intensity values for each fertility score using the mixed effects model. A scatter plot of unadjusted data points was superimposed on the regression line plot. Regression equations are shown for high fertility (y = −22,315× + 89,049) and low fertility (y = 9603× + 50,993) bulls

Back to article page