Fig. 4

Immunostaining of TH2B in low and high fertility bull spermatozoa. Intensity of TH2B in sperm from low (a) and high fertile bulls (b) using confocal microscopy. DNA stained with DAPI (blue) in a bull sperm; TH2B histone linked to FITC-conjugated secondary antibody (green) in bull sperm; merged images of DAPI and FITC; bright field images; and negative controls