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Table 3 PLM analysis of the oocyte and biochemical measurements in FF in relation to day 2 embryo morphology and progression to blastocyst

From: Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI

 

TQ embryos (n = 14)

PQ embryos (n = 9)

p

Blastocyst (n = 11)

Arrested (n = 18)

p

IL-ZP Retardance (nm)

1.7 ± 0.6

2.0 ± 0.7

ns

2.0 ± 0.2

2.0 ± 0.6

ns

IL-ZP Area (ÎĽm2)

2394 ± 652

2711 ± 632

ns

2768 ± 714

2762 ± 492

ns

IL-ZP Thickness (ÎĽm)

3.8 ± 1.5

4.8 ± 1.7

ns

5.1 ± 1.9

4.7 ± 1.2

ns

Total-ZP Thickness (ÎĽm)

17.3 ± 1.0

18.0 ± 2.0

ns

18.1 ± 2.1

18.4 ± 1.6

ns

Spindle Retardance (nm)

1.2 ± 0.5

1.6 ± 0.2

ns

1.4 ± 0.3

1.5 ± 0.3

ns

Spindle Area (ÎĽm2)

91.2 ± 30.7

85.5 ± 16.1

ns

72.2 ± 8.5

92.7 ± 21.0

p < 0.05

Spindle Major Axis (ÎĽm)

11.4 ± 2.7

11.4 ± 1.3

ns

9.6 ± 0.8

12.3 ± 1.4

p < 0.001

ffAMH (ng/ml)

1.7 ± 1.0

1.8 ± 1.1

ns

1.5 ± 0.9

2.0 ± 1.0

ns

LDH (pmol NADH/min/mg prot)

0.2 ± 0.1

0.2 ± 0.1

ns

0.2 ± 0.1

0.2 ± 0.1

ns

SOD (mlU/mg protein)

3.7 ± 0.9

3.9 ± 1.1

ns

4.2 ± 1.7

4.0 ± 0.9

ns

Catalase (mlU/mg protein)

9.5 ± 1.0

9.1 ± 1.0

ns

9.3 ± 0.3

9.4 ± 0.9

ns

  1. Top quality (TQ) embryos = day 2 morphological score ≥ 9; poor quality (PQ) embryos = day 2 morphological score ≤ 6.; Blastocyst = embryos reaching the blastocyst stage; Arrested = embryos who arrested their development. Values are expressed as mean ± standard deviation