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Table 3 PLM analysis of the oocyte and biochemical measurements in FF in relation to day 2 embryo morphology and progression to blastocyst

From: Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI

  TQ embryos (n = 14) PQ embryos (n = 9) p Blastocyst (n = 11) Arrested (n = 18) p
IL-ZP Retardance (nm) 1.7 ± 0.6 2.0 ± 0.7 ns 2.0 ± 0.2 2.0 ± 0.6 ns
IL-ZP Area (μm2) 2394 ± 652 2711 ± 632 ns 2768 ± 714 2762 ± 492 ns
IL-ZP Thickness (μm) 3.8 ± 1.5 4.8 ± 1.7 ns 5.1 ± 1.9 4.7 ± 1.2 ns
Total-ZP Thickness (μm) 17.3 ± 1.0 18.0 ± 2.0 ns 18.1 ± 2.1 18.4 ± 1.6 ns
Spindle Retardance (nm) 1.2 ± 0.5 1.6 ± 0.2 ns 1.4 ± 0.3 1.5 ± 0.3 ns
Spindle Area (μm2) 91.2 ± 30.7 85.5 ± 16.1 ns 72.2 ± 8.5 92.7 ± 21.0 p < 0.05
Spindle Major Axis (μm) 11.4 ± 2.7 11.4 ± 1.3 ns 9.6 ± 0.8 12.3 ± 1.4 p < 0.001
ffAMH (ng/ml) 1.7 ± 1.0 1.8 ± 1.1 ns 1.5 ± 0.9 2.0 ± 1.0 ns
LDH (pmol NADH/min/mg prot) 0.2 ± 0.1 0.2 ± 0.1 ns 0.2 ± 0.1 0.2 ± 0.1 ns
SOD (mlU/mg protein) 3.7 ± 0.9 3.9 ± 1.1 ns 4.2 ± 1.7 4.0 ± 0.9 ns
Catalase (mlU/mg protein) 9.5 ± 1.0 9.1 ± 1.0 ns 9.3 ± 0.3 9.4 ± 0.9 ns
  1. Top quality (TQ) embryos = day 2 morphological score ≥ 9; poor quality (PQ) embryos = day 2 morphological score ≤ 6.; Blastocyst = embryos reaching the blastocyst stage; Arrested = embryos who arrested their development. Values are expressed as mean ± standard deviation