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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI

Fig. 3

Western blotting analysis of 4-HNE-proteins conjugates in the FF. Panel a. Representative Western blot, obtained using antibody against anti-4-HNE-protein conjugates, of FF extracted proteins belonging to the F or nF groups. The 4-HNE-modified and non-modified non-patient human serum albumin (4-HNE-mod HSA and HSA) were used as positive and negative controls, respectively, to test the specificity of the method. The protein bands of the analyzed FFs were quantified by densitometry; the values, expressed as arbitrary units, are represented in Panel b as means ± standard error of the mean. F group = patients whose oocyte was fertilized (n = 29), nF group = patients whose oocyte was not (n = 17). *** p < 0.001

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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