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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI

Fig. 1

Representative images of PLM analysis of the oocyte. Automated detection by Oosight™ software of birefringent structures (in grey) of the oocyte (a); the ring-like structure and the ovoid region (in red) correspond to the automatic detection of the retardance, area and thickness of the inner layer of the zona pellucida (IL-ZP) and of the retardance and area of the meiotic spindle (MS) respectively (b); the total thickness of the ZP and the major axis length of the MS were manually measured using a line scan as depicted (c)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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