Table 1 The sequences of primers for quantitative fluorescent RT-PCR
Genes | Primers | Length of target fragment, bp |
|---|---|---|
β-Actin | F: 5′- ACTCCTATGTGGGTGACGAGG-3′ | 137 |
R: 5′-CACACGCAGCTCATTGTAGAAG-3′ | ||
NPY | F:5′- CCAGACAGAGATATGGCAAG-3′ | 110 |
R:5′- CATGGAAGGGTCTTCAAGCC-3′ | ||
PTPN11 | F:5′- TACGGGGTCATGCGTGTTAG-3′ | 125 |
R:5′- GAAAGTGGTACTGCCAGACG-3′ | ||
GYS1 | F:5′- TGGTGGGACCATACACGGA-3′ | 113 |
R:5′- TCAGCCAACGCCCAAAATAC-3′ | ||
PKLR | F:5′- GCCGCATCTACATTGACGAC-3′ | 127 |
R:5′- GCATTTGGCAAGTTCACACC-3′ | ||
F, Forward; R, reverse. | ||