Table 1 Oligonucleotide sequences of primers used for RT-qPCR analysis
| Transcript | Sequence (5′-3′ direction) | Gene accession no. | Product size (bp) | Efficiency |
|---|---|---|---|---|
| CHRDL1 |
AACAATGCCTGTGTATGAGT TCTGGGCTTCTCCTTCAGT | XM_005673817.2 | 242 | 91% |
| FST |
GAGCCCACCTCCTCAGGAC TCTCAGGGCACAGCTCATCG | NM_001003662.1 | 238 | 94% |
| TGFβR3 |
TGATCCACCATGAAGTGCAGT TGCCTTCCTGCGCTGTCTC | NM_214272.1 | 190 | 108% |
| ID1 |
AGCTGAACTCGGAATCCCAA TTCAGCGACACAAGATGCGAT | NM_001244700.1 | 147 | 107% |
| SMAD4 |
CCAAGTGCATATATAAAGGTCT AGCCTTTCACAAAACTCATCC | XM_013985326 | 235 | 98% |
| PBGD |
GAGAGTGCCCCTATGATGCT ATGATGGCACTGAACTCCT | NM_001097412.1 | 214 bp | 97% |
| B-ACTIN |
GGGAGATCGTGCGGGACAT CGTTGCCGATGGTGATGAC | DQ845171 | 141 bp | 99% |
| 18S rRNA |
GTGAAACTGCGAATGGCTC CCGTCGGCATGTATTAGCT | AB117609 | 105 bp | 97% |