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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Activating transcription factor 3 promotes embryo attachment via up-regulation of leukemia inhibitory factor in vitro

Fig. 5

Aberrantly low ATF3 expression in the endometria of RIF patients. a Timed midsecretory endometrial biopsies from women with fertilized embryos (FER) (control, n = 7) and repeated implantation failure (RIF) patients (n = 7) were analyzed for ATF3 protein expression using Western blot analysis. Biopsies from FER women (control, n = 13) and RIF patients (n = 15) were analyzed for ATF3 protein expression by Western blot analysis (normalized to the GAPDH protein expression level) (b) and LIF mRNA level by real-time PCR (c). *p < 0.05; **p < 0.01 vs. the control group. d Secretory endometrial tissue samples from fertile control and RIF patients are shown at 400× magnification. The negative control (NC) is nonspecific rabbit serum. Brown staining represents positive staining (arrows). Scale bars, 50 μm. e Correlation between ATF3 protein expression and LIF mRNA expression in endometrial samples of FER women and RIF patients

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