Fig. 2
Up-regulation of ATF3 in Ishikawa cells upon treatment with E2 and MPA. Ishikawa cells were treated with E2, MPA, or both E2 and MPA for the indicated time periods. ATF3 mRNA levels (a) were measured by real-time PCR. ATF3 protein expression (b) was determined by Western blot and was quantitated by densitometric analysis. *p < 0.05; **p < 0.01; ***p < 0.001 compared with untreated controls. c Pretreatment of ICI182780 and mifepristone was performed before sex hormones were analyzed. ATF3 protein expression was determined by Western blot. d Immunofluorescence images demonstrated endogenous ATF3 expression and F-actin demonstration in Ishikawa cells treated with E2 and MPA for 6 h. Scale bars, 25 μm