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Table 5 The effects of NPs on the developmental competence of immature porcine oocytes from 3.0-4.0 mm follicles (mean ± S. E. M.)

From: Natriuretic peptides improve the developmental competence of in vitro cultured porcine oocytes

  IVF PA
Oocytes (n) cleaved oocytes (%) blastocysts (%) Oocytes
(n)
cleaved oocytes (%) blastocysts (%)
Control 326 11.3 ± 1.9 a 4.2 ± 1.0 a 264 31.6 ± 2.5 a 12.6 ± 1.1 a
cilostamide 328 38.5 ± 1.7 b 8.8 ± 1.7 b 256 70.4 ± 3.7 b 25.6 ± 1.4b
BNP 314 40.5 ± 2.7 b 12.6 ± 2.5 c 311 81.1 ± 2.3 c 31.7 ± 2.3 c
CNP 289 41.4 ± 2.8 b 11.6 ± 1.6 c 288 78.4 ± 3.2c 32.0 ± 2.4 c
  1. Cleavage rates on day 2 and Blastocyst developmental rates on day 7 after IVF and PA of the COCs recovered from the 3.0 - 8.0 mm follicles in standard IVM for 46 h (as negative controls), or pre-cultured with PMCI–cilostamide 20 h plus IVM 46 h (as positive controls) and pre-cultured with PMCI–BNP/CNP 20 h plus IVM 46 h (as experimental groups). abcMeans in the same column with different superscripts were significantly different (p < 0.01)