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Table 5 The effects of NPs on the developmental competence of immature porcine oocytes from 3.0-4.0 mm follicles (mean ± S. E. M.)

From: Natriuretic peptides improve the developmental competence of in vitro cultured porcine oocytes

 

IVF

PA

Oocytes (n)

cleaved oocytes (%)

blastocysts (%)

Oocytes

(n)

cleaved oocytes (%)

blastocysts (%)

Control

326

11.3 ± 1.9 a

4.2 ± 1.0 a

264

31.6 ± 2.5 a

12.6 ± 1.1 a

cilostamide

328

38.5 ± 1.7 b

8.8 ± 1.7 b

256

70.4 ± 3.7 b

25.6 ± 1.4b

BNP

314

40.5 ± 2.7 b

12.6 ± 2.5 c

311

81.1 ± 2.3 c

31.7 ± 2.3 c

CNP

289

41.4 ± 2.8 b

11.6 ± 1.6 c

288

78.4 ± 3.2c

32.0 ± 2.4 c

  1. Cleavage rates on day 2 and Blastocyst developmental rates on day 7 after IVF and PA of the COCs recovered from the 3.0 - 8.0 mm follicles in standard IVM for 46 h (as negative controls), or pre-cultured with PMCI–cilostamide 20 h plus IVM 46 h (as positive controls) and pre-cultured with PMCI–BNP/CNP 20 h plus IVM 46 h (as experimental groups). abcMeans in the same column with different superscripts were significantly different (p < 0.01)