Skip to main content

Table 1 Variance of mPCR measurements over a range of 1–86% methylation

From: Association of skewed X-chromosome inactivation with FMR1 CGG repeat length and anti-Mullerian hormone levels: a cohort study

CGG#

Average % Methylation (n = 14)

Std Dev

18

3%

1%

30

59%

4%

32

5%

1%

56

38%

4%

85

1%

1%

116

86%

11%

>200

2%

1%

  1. Values near 50% methylation (38–59%) demonstrated standard deviations of 4%
  2. FMR1 alleles representing 7 distinct expansion lengths were assessed in 14 independent mPCR runs using multiple operators to establish the technical variation of the assay