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Table 1 Variance of mPCR measurements over a range of 1–86% methylation

From: Association of skewed X-chromosome inactivation with FMR1 CGG repeat length and anti-Mullerian hormone levels: a cohort study

CGG# Average % Methylation (n = 14) Std Dev
18 3% 1%
30 59% 4%
32 5% 1%
56 38% 4%
85 1% 1%
116 86% 11%
>200 2% 1%
  1. Values near 50% methylation (38–59%) demonstrated standard deviations of 4%
  2. FMR1 alleles representing 7 distinct expansion lengths were assessed in 14 independent mPCR runs using multiple operators to establish the technical variation of the assay