Fig. 4

Embryo temperature in the warming procedure was calculated using a thermal transfer-based simulation method. a-b: The temperature in the center of the embryo during the warming procedure was plotted using the simulation result for the Kitasato Vitrification System and the control device. The temperature changes are shown every 0.02 s from thawing solution immersion for 1 s (a) and every 0.002 s from immersion for 0.05 s (b)