Fig. 6
Down-regulation of StAR and CYP11A1 gene expression by steroid intermediates. Y1-BS1 cells were treated with or without HDL3 (30 μg/ml), 22(R)-diol (10 μM) or pregnenolone (10 μM) for different time. Cells were harvested, RNA isolated and reversed transcribed before subject to qRT-PCR analyses of StAR and CYP11A1 mRNA levels. 36B4 was used as internal control. Results are summary of three independent experiments with triplicates in each experiment. * p < 0.05, ** p < 0.01