Table 1 Details of the primers used for quantitative real-time RT-PCR analysis
Gene (GenBank accession number) | Primer | Sequence | Position |
|---|---|---|---|
CHGA | Forward | 5′-GCCGAAAGAGGTGACAGAAGA-3′ | 538-558 |
(NM_181005) | Reverse | 5′-GTCTCCGTCCGAGTCTTCATC-3′ | 637-617 |
CNGA1 | Forward | 5′-AGCAGAGATCGCCATCAATGT-3′ | 1574-1594 |
(NM_174278) | Reverse | 5′-ACCAACTCCACCAACAGACCA-3′ | 1663-1643 |
CPXM2 | Forward | 5′- ACCAGTGGATTGAAGTGGACG-3′ | 581-601 |
(NM_001206057) | Reverse | 5′- TCACTCAGCCAGAGTGAGTTCCT-3′ | 665-643 |
FAM83D | Forward | 5′- GGCTCCTACAGTTTTACATGGACAG-3′ | 788-812 |
(NM_001083393) | Reverse | 5′-CAACCACTTGGCCAGACAGAA-3′ | 863-843 |
FMO2 | Forward | 5′- AAGCCAGACATCCTTTCTCTCTTG -3′ | 1459-1482 |
(NM_001163274) | Reverse | 5′- CCCAACCAGGCGATACTGATA-3′ | 1554-1532 |
GSTA3 | Forward | 5′-AGAGCCATCCTCAGCTACCTTG-3′ | 254-275 |
(NM_001077112) | Reverse | 5′-TCGATCCTGACTGTCTCCTTCA-3′ | 327-306 |
IFIH1 | Forward | 5′-GGGACTAACAGCTTCACCAGGT-3′ | 1764-1785 |
(XM_002685338) | Reverse | 5′-GGTAACTGCATCAAGATTGGCA-3′ | 1860-1839 |
IGG1C | Forward | 5′-ACCAAGGTGGACAAGGCTGTT-3′ | 274-294 |
(S82409) | Reverse | 5′-GGAAGATGAAGACAGAGGGTCCT-3′ | 370-348 |
KCNA2 | Forward | 5′-TGGGTTCCCTATGTGCAATTG-3′ | 1644-1664 |
(NM_001101195) | Reverse | 5′-TCCCGGTGGTAGAAGTAGTTGAA-3′ | 1734-1712 |
KLHL24 | Forward | 5′- TTATTGGCAAGGAGGAGATGGT-3′ | 901-922 |
(NM_001206196) | Reverse | 5′- TCTCAGATCAACAGCGCGAT-3′ | 968-949 |
KRT35 | Forward | 5′- GAGACCGAGGTATCCATGCG-3′ | 587-606 |
(NM_001076073) | Reverse | 5′- TTCTTGAGGCAGAGCAGCTC -3′ | 726-707 |
LPLUNC1 | Forward | 5′- TCGGTGTGTTCAACCCTAAGC-3′ | 1280-1300 |
(NM_174697) | Reverse | 5′- TTCTCGTTTGGCAGCAGGAT -3′ | 1355-1336 |
PIPOX | Forward | 5′- ACAGCATTAACACCGAGTCGG-3′ | 2140-2160 |
(NM_001014878) | Reverse | 5′- GGCAGTTATGAGCCTGTTTCCT-3′ | 2210-2189 |
PLEKHA5 | Forward | 5′- GATGGATTCAAGAACGGAACG-3′ | 2655-2675 |
(XM_002687754) | Reverse | 5′- TTCCACAGTCATCCTAGGTCGA-3′ | 2739-2718 |
PRF1 | Forward | 5′-CAAGCCAAATGCTAATGTCCGT-3′ | 408-429 |
(NM_001143735) | Reverse | 5′-AAAGCGACACTCCACTAAGTCCAT-3′ | 531-508 |
PRSS2 | Forward | 5′-GTGAGGCTGGGAGAATACAACA-3′ | 211-232 |
(NM_174690) | Reverse | 5′-ATGATCTTGGACGCATCGATGA-3′ | 281-260 |
SLC39A2 | Forward | 5′- TTGGCTGCCTATTTGCCCT-3′ | 355-373 |
(NM_001205648) | Reverse | 5′- CTGGAACCACTTGAAGCAGATG-3′ | 428-407 |
THBS4 | Forward | 5′- CACTCTGAACGAGCTCTACGTGAT 3′ | 331-354 |
(NM_001034728) | Reverse | 5′- GAAGAGTAAAGGCCGAAGATGGT-3′ | 411-389 |
SUZ12 | Forward | 5′-GAACACCTATCACACACATTCTTGT-3′ | 1565-1589 |
(NM_001205587) | Reverse | 5′-TAGAGGCGGTTGTGTCCACT-3′ | 1694-1675 |