Table 2 Primer sequences used in quantitative real-time PCR in human and mouse specimen
Gene | Sense primer 5’ –3’ | Antisense primer 5’ –3’ |
|---|---|---|
GAPDH (human) | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTGATGAG |
SOX2 (human) | TACAGCATGTCCTACTCGCAG | GAGGAAGAGGTAACCACAGGG |
NANOG (human) | AAGGTCCCGGTCAAGAAACAG | CTTCTGCGTCACACCATTGC |
OCT4 (human) | GCAGCGACTATGCACAACGA | CCAGAGTGGTGACGGAGACA |
GAPDH (mouse) | TGACCTCAACTACATGGTCTACA | CTTCCCATTCTCGGCCTTG |
SOX2 (mouse) | GCGGAGTGGAAACTTTTGTCC | GGGAAGCGTGTACTTATCCTTCT |
NANOG (mouse) | CACAGTTTGCCTAGTTCTGAGG | GCAAGAATAGTTCTCGGGATGAA |
OCT4 (mouse) | CGGAAGAGAAAGCGAACTAGC | ATTGGCGATGTGAGTGATCTG |