Fig. 6

Effect of REAC treatment on TEAC (trolox equivalent antioxidant capacity) in the cellular extract of stallion spermatozoa (panel a) and in the media (panel b) during 72 h. of storage at 4 °C. For each parameter, panels a and b show the mean ± S.E values determined at the different time points. A total of 16 ejaculates collected from eight stallions of different breeds (1: Thoroughbred; 2, 3, 4, 6: Arabian; 5, 7, 8: Warmblood) were used. Asterisks indicate statistical differences between REAC treated and untreated controls (General Linear Model): * p > 0.05; ** p < 0.001. Panel b Different letters indicate a statistical difference among values recorded at the different time points within the same experimental group (General Linear Model): p < 0.001. Upper case letters: REAC group; lower case letters: control group