Fig. 3
From: REAC technology as optimizer of stallion spermatozoa liquid storage
Effect of REAC treatment on acrosome integrity, as evaluated by FITC-PSA staining, of stallion spermatozoa during 72 h. of storage at 4 °C. Panels a and b show the mean ± S.E values determined at the different time points. Panel A shows the percentage of spermatozoa with intact acrosome, while panel b shows the percentage of spermatozoa with damaged acrosome. A total of 16 ejaculates collected from eight stallions of different breeds (1: Thoroughbred; 2, 3, 4, 6: Arabian; 5, 7, 8: Warmblood) were used. The microscopic images show a live spermatozoon with intact acrosome (1; no fluorescent staining), a live spermatozoon with damaged acrosome (2; FITC-PSA positive), and a dead spermatozoon with damaged acrosome (3; PI and FITC-PSA positive), identified using fluorescein isothiocynatelabeledPisumSativum agglutinin (FITC-PSA) and propidium iodide (PI). Asterisks indicate statistical differences between REAC treated and untreated controls (General Linear Model): p > 0.001. Different letters indicate a statistical difference among values recorded at the different time points within the same experimental group (General Linear Model): p > 0.01. Upper case letters: REAC group; lower case letters: control group