Fig. 2

G-CSF-enhanced spermatogenic recovery after busulfan treatment is stable. Results are from animals in Experiment 1 (a) Testis weights (mean ± standard error). White numbers at base of bars indicate animal numbers in each group. b Stacked bars show the percentage of all seminiferous tubule cross-sections counted from all animals in each group which exhibit differing degrees of spermatogenesis: complete spermatogenesis (complete), up to round spermatids (Rnd Std), up to 1° spermatocytes (1° Sct), or containing no spermatogenesis (Empty or Sertoli cell-only). A, B, and C categorical notations above bars denote statistically significant differences between groups (p <0.05) as determined by t-tests. Histology results were similar to previous examples shown in our prior work [17]. The number of seminiferous tubule cross-sections evaluated per animal is shown in Additional file 1: Table S1