Fig. 6From: Human LH and hCG stimulate differently the early signalling pathways but result in equal testosterone synthesis in mouse Leydig cells in vitroEvaluation of testosterone production upon LH or hCG stimulation in the presence or absence of specific inhibitors, in mouse Leydig cells. The cells were stimulated 24 h by EC80 LH and hCG, in the presence or absence of H-89 or U0126. Total testosterone levels were measured by immune-assay and represented as means ± SEM (§ = significant vs “unstimulated, no inhibitors”; * = significant LH/hCG vs corresponding LH/hCG samples without inhibitor; Mann-Whitney’s U-test; P < 0.05; n = 4)Back to article page