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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Human LH and hCG stimulate differently the early signalling pathways but result in equal testosterone synthesis in mouse Leydig cells in vitro

Fig. 4

Comparison between LH- and hCG-induced testosterone production in murine primary Leydig cells. The steroid was measured in 24 h-stimulated cells by increasing LH and hCG doses, in the presence of 500 μM IBMX. a Total testosterone levels was measured by immuno-assay and represented as means ± SEM in ng/ml, then non-linear regressions were plotted. b Testosterone levels were normalized in percentage of the maximal response. EC50 values were statistically compared (hCG EC50 = 36.66 ± 12.25 pM; LH EC50 = 50.08 ± 18.03 pM; means ± SEM; Mann-Whitney’s U-test; P = 1.00; n = 5)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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