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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Human LH and hCG stimulate differently the early signalling pathways but result in equal testosterone synthesis in mouse Leydig cells in vitro

Fig. 2

Evaluation of pErk1/2 and pCreb activation upon LH and hCG treatment. a Mouse Leydig cells were stimulated by increasing doses of LH and hCG. The phosphorylation of Erk1/2 and Creb was evaluated after 15 min by Western blotting (image representative of five independent experiments). b, c Densitometric analysis of pErk1/2 (b) and pCreb (c) signals. The values were normalized over total ERK and represented as means ± SEM, then statistically evaluated (§ = significant vs control; * = significant LH vs hCG; two-way ANOVA and Bonferroni post-tests; P < 0.05; n = 5)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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