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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Human LH and hCG stimulate differently the early signalling pathways but result in equal testosterone synthesis in mouse Leydig cells in vitro

Fig. 1

Comparison between LH- and hCG-induced cAMP production by dose–response experiment. Murine primary Leydig cells were stimulated by increasing doses of hCG and LH, in the presence of 500 μM IBMX. Total cAMP was measured after 3 h of incubation and cAMP levels were normalized as percentage of the maximal response. All the results are represented as means ± SEM in a logarithmic X-axis, then non-linear regressions were plotted. The EC50 cAMP values were compared (LH EC50 = 192 ± 53.96 pM; hCG EC50 = 18.64 ± 10.14 pM; means ± SEM; Mann-Whitney’s U-test; P = 0.0286; n = 4)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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