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Table 3 Comparison of qPCR and microarray data from GC cultured under high vs. normal density culture conditions

From: Induction of altered gene expression profiles in cultured bovine granulosa cells at high cell density

Gene symbol Gene name FC qRT-PCR FC microarray Correlation coefficient
CYP19A1 cytochrome P450, family 19, subfamily A, polypeptide 1, aromatase −11.09 −7.02 0.99
CYP11A1 cytochrome P450, family 11, subfamily A, polypeptide 1 1.65 1.24a 0.98
HSD3B1 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta −2.43 −1.2a 0.98
FSHR follicle stimulating hormone receptor −5.97 −4.64 0.99
LHCGR luteinizing hormone/choriogonadotropin receptor −4.12 −2.35 0.98
PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase) 1.09a 1.27a 0.78
RGS2 regulator of G-protein signaling 2 4.84 3.3 0.99
VNN2 vanin 2 7.75 5.88 0.99
PTX3 pentraxin 3, long 3.95 3.02 0.96
PCNA proliferating cell nuclear antigen −1.16a −1.18a 0.83
CCND2 cyclin D2 −1.69 −1.37a 0.96
  1. FC fold change, qRT-PCR was normalized to the reference gene RPLP0; microarray data was normalized with the RMA method; all correlations were significant with P < 0.05; genes labelled with awere not classified as significant according to microarray analysis, because the FC did not reach the threshold of 1.5 or −1.5