Induction of altered gene expression profiles in cultured bovine granulosa cells at high cell density

Table 3 Comparison of qPCR and microarray data from GC cultured under high vs. normal density culture conditions

Gene symbol	Gene name	FC qRT-PCR	FC microarray	Correlation coefficient
CYP19A1	cytochrome P450, family 19, subfamily A, polypeptide 1, aromatase	−11.09	−7.02	0.99
CYP11A1	cytochrome P450, family 11, subfamily A, polypeptide 1	1.65	1.24^a	0.98
HSD3B1	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta	−2.43	−1.2^a	0.98
FSHR	follicle stimulating hormone receptor	−5.97	−4.64	0.99
LHCGR	luteinizing hormone/choriogonadotropin receptor	−4.12	−2.35	0.98
PTGS2	prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase)	1.09^a	1.27^a	0.78
RGS2	regulator of G-protein signaling 2	4.84	3.3	0.99
VNN2	vanin 2	7.75	5.88	0.99
PTX3	pentraxin 3, long	3.95	3.02	0.96
PCNA	proliferating cell nuclear antigen	−1.16^a	−1.18^a	0.83
CCND2	cyclin D2	−1.69	−1.37^a	0.96

FC fold change, qRT-PCR was normalized to the reference gene RPLP0; microarray data was normalized with the RMA method; all correlations were significant with P < 0.05; genes labelled with ^awere not classified as significant according to microarray analysis, because the FC did not reach the threshold of 1.5 or −1.5

ISSN: 1477-7827