ACTH-induced stress in weaned sows impairs LH receptor expression and steroidogenesis capacity in the ovary

Table 1 Sequences and sizes of all tested genes

Gene	Reference sequence	Primer sequence (5’-3’)	Size
LHR	NM-214449.1	sense GCTCACCCAAGACACTCC	190
LHR	NM-214449.1	antisense CACATGAGGAAACGAGGC	190
StAR	NM-213755.2	sense GGCAAGGCTCTTCTAACT	99
StAR	NM-213755.2	antisense TAGACACGAAAGGGCTCA	99
P450scc	NM-214427.11	sense CCAGCATTACCAGAAGCC	92
P450scc	NM-214427.11	antisense GAGCCATTACCTCCGTGT	92
P450arom	NM-214429.1	sense AAGAAGGGTCACAACAAG	165
P450arom	NM-214429.1	antisense AAGAAAGCCAGTGAGCAG	165
P450c17	NM-214428.1	sense ATGATCCAAGCCAAGACG	140
P450c17	NM-214428.1	antisense TTTACCACAGAGGCAGAAG	140
3β-HSD	NM-001004049.1	sense CCTGGCAAGTATTTCTCGG	107
3β-HSD	NM-001004049.1	antisense CCAGCAACAAGTGGACGAT	107
β-actin	NM-001167795.1	sense CTCCATCATGAAGTGCGACGT	114
β-actin	NM-001167795.1	antisense GTGATCTCCTTCTGCATCCTGTC	114

ISSN: 1477-7827