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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Cryopreservation and xenografting of human ovarian fragments: medulla decreases the phosphatidylserine translocation rate

Fig. 3

Translocation of phosphatidylserine in ovarian tissue frozen in form of medulla-free and medulla-contained strips and then xenografted in SCID mice: representative example of one experiment. a, b, c, d, e tissue frozen in form of medulla-free cortex, f, g, h, i, j tissue frozen in form of medulla-contained cortex, a, f forward and scatter dot plot used to select the interest population, c, h histograms displaying fluorescence of PE channel, used to measure fluorescence intensity of Propidium Iodide (PI), (D, I) dot plot analysis of FITC-Annexin V and PE channels, (Q1) cells negative to Annexin V (FITC A) and positive to PI (could indicate necrotic cells), (Q2) cells positive to both Annexin V and PI (could indicate late apoptotic stage), (Q3) cells negative for both Annexin V and PI (could indicate viable cells), (Q4) cells positive to FITC-Annexin V and negative to PI (could indicate early apoptotic state)

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