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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer

Fig. 4

Phenotype detection. a Comparison of birth weight between cloned GTKO piglets and the control. b Flow cytometric analysis of GTKO pigs with FITC-conjugated GS-IB4 lectin staining. c Confocal microscopy analysis of fibroblasts from GTKO piglets stained with FITC-conjugated GS-IB4. d Immunochemical analysis of the GTKO pig kidney. Wild-type Diannan miniature pigs were used as the positive control. e Protein expression levels were assessed via Western blotting. GGTA1 protein expression in the pancreas tissue of GTKO and WT pig are shown in cropped blots using an anti-GGTA1 monoclonal antibody. Anti-β-actin served as a loading control

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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